Induction of high affinity IL 2 receptors on B cells responding to anti-Ig and T cell-derived helper factors

S. Prakash, R. J. Robb, R. D. Stout, D. C. Parker

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We have reported that IL 2 is one of the essential helper factors in culture supernatants from concanavalin A-activated spleen cells or T cell hybridomas that support proliferation and immunoglobulin secretion in B cell cultures responding to anti-immunoglobulin. Here we show that cells in such cultures consume IL 2 and bear high affinity IL 2 receptors detected by binding of purified, radiolabeled IL 2. Induction of high affinity IL 2 receptors depends on addition of both anti-immunoglobulin and helper factors, and does not occur in cultures given only anti-Ig, only helper factors, concanavalin A plus helper factors, or LPS. The majority of IL 2 receptors are on cells that also bear endogenous membrane immunoglobulin, because they are found in the membrane immunoglobulin-positive fraction when cultured cells are separated by fluorescence sorting after overnight culture in the absence of anti-immunoglobulin to allow reexpression of membrane immunoglobulin.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalJournal of Immunology
Volume135
Issue number1
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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