Induction of elongation in cultured rat lens epithelial cells by FGF and inhibition by selenite

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Abstract

The purposes of this experiment were to: (1) test if fibroblast growth factor (FGF) induced elongation of cultured rat lens epithelial cells (LEC) and (2) determine if selenite affected elongation of LEC. FGF (125-500 ng/ml) reduced the number of colonies of LEC, but it did not induce elongation when cells were cultured on plastic dishes. One hundred micromolar and, to a lesser extent, 10 μmol/l selenite also reduced the number of colonies of LEC. Coculture of FGF and selenite on plastic caused a synergistic reduction in the number of colonies. FGF (125-1000 ng/ml) induced a dramatic morphologic change in LEC. Elongated processes radiated from stellate-like cell aggregates when cells were cultured on reconstituted basement membrane matrix (Matrigel). Again, 100 μmol/l selenite and, to a lesser extent, 10 μmol/l selenite reduced the number of cell aggregates with processes on Matrigel. These results indicated that an inhibitory effect of selenite on the elongation of LEC may be a factor in the development of selenite cortical cataract.

Original languageEnglish (US)
Pages (from-to)2528-2531
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume33
Issue number8
StatePublished - 1992

Fingerprint

Selenious Acid
Fibroblast Growth Factors
Lenses
Epithelial Cells
Plastics
Cultured Cells
Fibroblast Growth Factor 1
Coculture Techniques
Basement Membrane
Cataract
Cell Count

Keywords

  • elongation
  • extracellular matrix
  • FGF
  • lens epithelial cell
  • rat
  • selenite

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Induction of elongation in cultured rat lens epithelial cells by FGF and inhibition by selenite",
abstract = "The purposes of this experiment were to: (1) test if fibroblast growth factor (FGF) induced elongation of cultured rat lens epithelial cells (LEC) and (2) determine if selenite affected elongation of LEC. FGF (125-500 ng/ml) reduced the number of colonies of LEC, but it did not induce elongation when cells were cultured on plastic dishes. One hundred micromolar and, to a lesser extent, 10 μmol/l selenite also reduced the number of colonies of LEC. Coculture of FGF and selenite on plastic caused a synergistic reduction in the number of colonies. FGF (125-1000 ng/ml) induced a dramatic morphologic change in LEC. Elongated processes radiated from stellate-like cell aggregates when cells were cultured on reconstituted basement membrane matrix (Matrigel). Again, 100 μmol/l selenite and, to a lesser extent, 10 μmol/l selenite reduced the number of cell aggregates with processes on Matrigel. These results indicated that an inhibitory effect of selenite on the elongation of LEC may be a factor in the development of selenite cortical cataract.",
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T1 - Induction of elongation in cultured rat lens epithelial cells by FGF and inhibition by selenite

AU - Azuma, M.

AU - Shearer, Thomas (Tom)

PY - 1992

Y1 - 1992

N2 - The purposes of this experiment were to: (1) test if fibroblast growth factor (FGF) induced elongation of cultured rat lens epithelial cells (LEC) and (2) determine if selenite affected elongation of LEC. FGF (125-500 ng/ml) reduced the number of colonies of LEC, but it did not induce elongation when cells were cultured on plastic dishes. One hundred micromolar and, to a lesser extent, 10 μmol/l selenite also reduced the number of colonies of LEC. Coculture of FGF and selenite on plastic caused a synergistic reduction in the number of colonies. FGF (125-1000 ng/ml) induced a dramatic morphologic change in LEC. Elongated processes radiated from stellate-like cell aggregates when cells were cultured on reconstituted basement membrane matrix (Matrigel). Again, 100 μmol/l selenite and, to a lesser extent, 10 μmol/l selenite reduced the number of cell aggregates with processes on Matrigel. These results indicated that an inhibitory effect of selenite on the elongation of LEC may be a factor in the development of selenite cortical cataract.

AB - The purposes of this experiment were to: (1) test if fibroblast growth factor (FGF) induced elongation of cultured rat lens epithelial cells (LEC) and (2) determine if selenite affected elongation of LEC. FGF (125-500 ng/ml) reduced the number of colonies of LEC, but it did not induce elongation when cells were cultured on plastic dishes. One hundred micromolar and, to a lesser extent, 10 μmol/l selenite also reduced the number of colonies of LEC. Coculture of FGF and selenite on plastic caused a synergistic reduction in the number of colonies. FGF (125-1000 ng/ml) induced a dramatic morphologic change in LEC. Elongated processes radiated from stellate-like cell aggregates when cells were cultured on reconstituted basement membrane matrix (Matrigel). Again, 100 μmol/l selenite and, to a lesser extent, 10 μmol/l selenite reduced the number of cell aggregates with processes on Matrigel. These results indicated that an inhibitory effect of selenite on the elongation of LEC may be a factor in the development of selenite cortical cataract.

KW - elongation

KW - extracellular matrix

KW - FGF

KW - lens epithelial cell

KW - rat

KW - selenite

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