TY - JOUR
T1 - Induction of both cytosolic phospholipase A2 and prostaglandin H synthase-2 by interleukin-1β in WISH cells is inhibited by dexamethasone
AU - Xue, Song
AU - Slater, Donna M.
AU - Bennett, Phillip R.
AU - Myatt, Leslie
N1 - Funding Information:
supported by NIH grants HD 26167 and should be directed to Leslie Myatt, Ph.D., and Gynecology, niversity of Cincinnati Box 670526, Cincinnati, Ohio 45267-0526.
PY - 1996/2
Y1 - 1996/2
N2 - In previous studies we have shown that IL- 1β induced both PGE2 release and total cellular cPLA2 activity and cPLA2 protein synthesis in human amnion-derived WISH cells. In this study, the effect of IL-1β on cPLA2 and PGHS-2 mRNA expression was investigated. Using RT-PCR, we found that IL-1β (0.1 ng/ml) coordinately induced both cPLA2 and PGHS-2 mRNA expression within 2 hours. The synthetic glucocorticoid dexamethasone (10-10-10-6M) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression activity and protein synthesis and PGE2 release in a concentration dependent manner. In the absence of IL-1β, dexamethasone alone (10-6M) inhibited basal CPLA2 activity, mRNA expression and protein synthesis. In addition, cycloheximide (5 μg/ml) apparently superinduced, but actinomycin D (2 μg/ml) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression suggesting that both are immediate early genes and a transcriptional mechanism is involved in the induction of both CPLA2 and PGHS-2 mRNA by IL-1beta;.
AB - In previous studies we have shown that IL- 1β induced both PGE2 release and total cellular cPLA2 activity and cPLA2 protein synthesis in human amnion-derived WISH cells. In this study, the effect of IL-1β on cPLA2 and PGHS-2 mRNA expression was investigated. Using RT-PCR, we found that IL-1β (0.1 ng/ml) coordinately induced both cPLA2 and PGHS-2 mRNA expression within 2 hours. The synthetic glucocorticoid dexamethasone (10-10-10-6M) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression activity and protein synthesis and PGE2 release in a concentration dependent manner. In the absence of IL-1β, dexamethasone alone (10-6M) inhibited basal CPLA2 activity, mRNA expression and protein synthesis. In addition, cycloheximide (5 μg/ml) apparently superinduced, but actinomycin D (2 μg/ml) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression suggesting that both are immediate early genes and a transcriptional mechanism is involved in the induction of both CPLA2 and PGHS-2 mRNA by IL-1beta;.
UR - http://www.scopus.com/inward/record.url?scp=0029879242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029879242&partnerID=8YFLogxK
U2 - 10.1016/0090-6980(95)00182-4
DO - 10.1016/0090-6980(95)00182-4
M3 - Article
C2 - 8711133
AN - SCOPUS:0029879242
SN - 0090-6980
VL - 51
SP - 107
EP - 124
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
IS - 2
ER -