Induction of both cytosolic phospholipase A2 and prostaglandin H synthase-2 by interleukin-1β in WISH cells is inhibited by dexamethasone

Song Xue, Donna M. Slater, Phillip R. Bennett, Leslie Myatt

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

In previous studies we have shown that IL- 1β induced both PGE2 release and total cellular cPLA2 activity and cPLA2 protein synthesis in human amnion-derived WISH cells. In this study, the effect of IL-1β on cPLA2 and PGHS-2 mRNA expression was investigated. Using RT-PCR, we found that IL-1β (0.1 ng/ml) coordinately induced both cPLA2 and PGHS-2 mRNA expression within 2 hours. The synthetic glucocorticoid dexamethasone (10-10-10-6M) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression activity and protein synthesis and PGE2 release in a concentration dependent manner. In the absence of IL-1β, dexamethasone alone (10-6M) inhibited basal CPLA2 activity, mRNA expression and protein synthesis. In addition, cycloheximide (5 μg/ml) apparently superinduced, but actinomycin D (2 μg/ml) inhibited IL-1β-induced cPLA2 and PGHS-2 mRNA expression suggesting that both are immediate early genes and a transcriptional mechanism is involved in the induction of both CPLA2 and PGHS-2 mRNA by IL-1beta;.

Original languageEnglish (US)
Pages (from-to)107-124
Number of pages18
JournalProstaglandins and Other Lipid Mediators
Volume51
Issue number2
DOIs
StatePublished - Feb 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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