Induction of B cell lymphomas by overexpression of a Myb oncogene truncated at either terminus

Richard Press, Todd W. Wisner, Donald L. Ewert

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The c-myb oncogene encodes a nuclear transcriptional transactivator that is often terminally truncated in hematopoietic tumors. To directly assess the tumorigenic activity of full length and terminally-truncated variants of c-myb, we have overexpressed several structurally-altered forms of myb within an avian retroviral vector and have shown that overexpression of truncated (but not full length) myb transforms both myeloid cells in vitro and mesenchymal cells in vivo. In vivo infection with these truncated myb viruses is now shown to induce metastatic B cell lymphomas in a significant minority of animals. Evaluation of the lymphomas revealed two distinct mechanisms of myb-induced tumorigenesis. In most of the lymphomas, proviral DNA inserted into the endogenous chicken c-myb gene and promoted the expression of a 5'-truncated myb transcript encoding an amino terminal truncated protein. In comparison, some animals infected with a virus encoding a carboxyl (C) terminal truncated myb (T-myb) developed non-insertional B cell lymphomas that directly expressed the provirally-encoded T-myb gene. The lymphomagenic T-myb protein lacks 214 C terminal amino acids including all of the myb transcription inhibition domain. This novel lymphomagenic activity for a C terminal truncated myb suggests that a loss of regulatory sequences at either end of c-myb is sufficient to create a B cell-specific transforming gene.

Original languageEnglish (US)
Pages (from-to)525-535
Number of pages11
JournalOncogene
Volume11
Issue number3
StatePublished - Aug 3 1995

Fingerprint

myb Genes
B-Cell Lymphoma
Lymphoma
Viruses
Trans-Activators
Myeloid Cells
Oncogenes
Chickens
Carcinogenesis
Proteins
B-Lymphocytes
Gene Expression
Amino Acids
DNA
Infection
Neoplasms

Keywords

  • B cell
  • Lymphoma
  • myb
  • Oncogene

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Induction of B cell lymphomas by overexpression of a Myb oncogene truncated at either terminus. / Press, Richard; Wisner, Todd W.; Ewert, Donald L.

In: Oncogene, Vol. 11, No. 3, 03.08.1995, p. 525-535.

Research output: Contribution to journalArticle

Press, Richard ; Wisner, Todd W. ; Ewert, Donald L. / Induction of B cell lymphomas by overexpression of a Myb oncogene truncated at either terminus. In: Oncogene. 1995 ; Vol. 11, No. 3. pp. 525-535.
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