Introduction of hybrid plasmids, which were constructed by ligation of pCRl or pMNl vector plasmid and SalI restriction endonuclease cleaved segments of Streptomyces cacaoi chromosome, resulted in the production of new β-lactamase and penicillin-binding protein in Escherichia coli. The, 5-lactamase and penicillin-binding protein were not from S. cacaoi but rather induced by the plasmids. Close relationship was observed between plasmids and penicillin-binding proteins but not with, 5-lactamase.
ASJC Scopus subject areas
- Drug Discovery