Introduction of hybrid plasmids, which were constructed by ligation of pCR1 or pMN1 vector plasmid and Sa/I restriction endonuclease cleaved segments of Streptomyces cacaoi chromosome, resulted in the production of new β-lactamase and penicillin-binding protein in Escherichia coli. The β-lactamase and penicillin-binding protein were not from S. cacaoi but rather induced by the plasmids. Close relationship was observed between plasmids and penicillin-binding proteins but not with β-lactamase.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Antibiotics|
|Publication status||Published - 1983|
ASJC Scopus subject areas
- Molecular Medicine