Induction of β-lactamase and penicillin-binding proteins in Escherichia coli by introduction of Streptomyces DNA

H. Nakazawa, Michiko Nakano, H. Ogawara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction of hybrid plasmids, which were constructed by ligation of pCR1 or pMN1 vector plasmid and Sa/I restriction endonuclease cleaved segments of Streptomyces cacaoi chromosome, resulted in the production of new β-lactamase and penicillin-binding protein in Escherichia coli. The β-lactamase and penicillin-binding protein were not from S. cacaoi but rather induced by the plasmids. Close relationship was observed between plasmids and penicillin-binding proteins but not with β-lactamase.

Original languageEnglish (US)
Pages (from-to)1543-1548
Number of pages6
JournalJournal of Antibiotics
Volume36
Issue number11
StatePublished - 1983
Externally publishedYes

Fingerprint

Penicillin-Binding Proteins
Streptomyces
Plasmids
Escherichia coli
DNA
DNA Restriction Enzymes
Ligation
Chromosomes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Induction of β-lactamase and penicillin-binding proteins in Escherichia coli by introduction of Streptomyces DNA. / Nakazawa, H.; Nakano, Michiko; Ogawara, H.

In: Journal of Antibiotics, Vol. 36, No. 11, 1983, p. 1543-1548.

Research output: Contribution to journalArticle

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