Induction by transforming growth factor β of pemphigus vulgaris antigen activity in mouse papilloma cells

Molly Kulesz-Martin, Ingrid Glurich, Barbara Lisafeld, Paul Kozlowski

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Abstract

Induction of a marker of epidermal spinous cells, pemphigus antigen activity, was detected by indirect immunofluorescence in murine papilloma cells exposed to human transforming growth factor β (TGF-β). Detection of pemphigus antigen activity required exposure of cells to 1.4 mM Ca2+ for 3 h just prior to immunoassay. The brief exposure to Ca2+ may be necessary for translocation of intracellular pemphigus antigen to the cell surface, where it is accessible to antibody. Cells grown in medium containing 0.02-0.04 mM Ca2+ were shown previously to be primarily basal cells characterized by pemphigoid antigen activity. Following treatment with 0.25-25 pg/ml TGF-β for 44 h under 0.02-0.04 mM Ca2+ conditions, 63 ± 9% (SD) of cells were pemphigus positive. This percentage was comparable to that of positive control cultures exposed to 1.4 mM Ca2+ for 44 h (70 ± 10%) and was up to 2-fold that of solvent control cultures. Pemphigus antigen activity was significantly induced by 0.1-25 pg/ml TGF-β, out of a tested range of 10-5-103 pg/ml. The total number of papilloma cell colonies was unaffected by treatment with 0.1-25 pg/ml TGF-β but was reduced greater than 90% by treatment with 103-5 × 103 pg/ml TGF-β. The described immunofluorescence assay for pemphigus antigen activity may be useful for preliminary evaluation of differentiation-inducing agents in anticarcinoma therapy.

Original languageEnglish (US)
Pages (from-to)686-690
Number of pages5
JournalCancer Research
Volume50
Issue number3
Publication statusPublished - 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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