Inducible cell contact signals regulate early activation gene expression during B-T lymphocyte collaboration

Stephen J. Klaus, David Parker

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

B cells get help in the antibody response by presenting processed Ag to Th cells. We asked whether the Ag-presenting B cell must induce Th functions before receiving help, or whether B cell activation is a direct consequence of T cell recognition of Ag on the B cell surface. To obtain a prompt and sensitive indication of the receipt of growth signals, we measured mRNA levels of the immediate early genes, c-myc and egr-1, in T and B cells separated from Ag-specific B-T conjugates of normal resting murine B cells and a Th line. Although Ag-dependent increases in B cell c-myc expression occur as early as 2 h after conjugation, early c-myc expression in the B cell was also seen when the Th cells were activated with immobilized anti-CD3 in the absence of Ag recognition. Therefore, T cell activation rather than Ag recognition per se appears to be responsible for the early c-myc signal in the B cells. The c-myc response in the B cell depends on induction of a contact-dependent helper function in the T cell, which is inhibitable by cyclosporin A acting on the T cell. Delivery of contact help is not blocked by anti-class II MHC antibody. Contact with activated Th cells induces a different pattern of immediate early gene expression from that induced by cross-linking the B cell Ag receptor.

Original languageEnglish (US)
Pages (from-to)1867-1875
Number of pages9
JournalJournal of Immunology
Volume149
Issue number6
StatePublished - Sep 15 1992
Externally publishedYes

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B-Lymphocytes
T-Lymphocytes
Gene Expression
Immediate-Early Genes
Cyclosporine
Antibody Formation
Messenger RNA
Antibodies
Growth

ASJC Scopus subject areas

  • Immunology

Cite this

Inducible cell contact signals regulate early activation gene expression during B-T lymphocyte collaboration. / Klaus, Stephen J.; Parker, David.

In: Journal of Immunology, Vol. 149, No. 6, 15.09.1992, p. 1867-1875.

Research output: Contribution to journalArticle

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