Induced fit in guanidino kinases - Comparison of substrate-free and transition state analog structures of arginine kinase

Mohammad S. Yousef, Shawn A. Clark, Pamela K. Pruett, Thayumanasamy Somasundaram, W. Ross Ellington, Michael S. Chapman

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Arginine kinase (AK) is a member of the guanidino kinase family that plays an important role in buffering ATP concentration in cells with high and fluctuating energy demands. The AK specifically catalyzes the reversible phosphoryl transfer between ATP and arginine. We have determined the crystal structure of AK from the horseshoe crab (Limulus polyphemus) in its open (substrate-free) form. The final model has been refined at 2.35 Å with a final R of 22.3% (Rfree = 23.7%). The structure of the open form is compared to the previously determined structure of the transition state analog complex in the closed form. Classically, the protein would be considered two domain, but dynamic domain (DynDom) analysis shows that most of the differences between the two structures can be considered as the motion between four rigid groups of nonsequential residues. ATP binds near a cluster of positively charged residues of a fixed dynamic domain. The other three dynamic domains close the active site with separate hinge rotations relative to the fixed domain. Several residues of key importance for the induced motion are conserved within the phosphagen kinase family, including creatine kinase. Substantial conformational changes are induced in different parts of the enzyme as intimate interactions are formed with both substrates. Thus, although induced fit occurs in a number of phosphoryl transfer enzymes, the conformational changes in phosphagen kinases appear to be more complicated than in prior examples.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalProtein Science
Issue number1
StatePublished - Jan 1 2003
Externally publishedYes


  • Arginine kinase
  • Creatine kinase
  • Guanidino kinase
  • Induced fit
  • Structure

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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