Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer

Minna M. Tanner, Mika Tirkkonen, Anne Kallioniemi, Jorma Isola, Tuula Kuukasjärvi, Colin Collins, David Kowbel, Xin Yuan Guan, Jeff Trent, Joe Gray, Paul Meltzer, Olli P. Kallioniemi

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6% of the cases), PTPN1 region 3 Mb proximal (6.2%), and A1B3 region at 20q11 (6.2%). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co- amplification in breast cancer.

Original languageEnglish (US)
Pages (from-to)3441-3445
Number of pages5
JournalCancer Research
Volume56
Issue number15
Publication statusPublished - Aug 1 1996
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tanner, M. M., Tirkkonen, M., Kallioniemi, A., Isola, J., Kuukasjärvi, T., Collins, C., ... Kallioniemi, O. P. (1996). Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. Cancer Research, 56(15), 3441-3445.