Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer

Minna M. Tanner, Mika Tirkkonen, Anne Kallioniemi, Jorma Isola, Tuula Kuukasjärvi, Colin Collins, David Kowbel, Xin Yuan Guan, Jeff Trent, Joe Gray, Paul Meltzer, Olli P. Kallioniemi

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6% of the cases), PTPN1 region 3 Mb proximal (6.2%), and A1B3 region at 20q11 (6.2%). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co- amplification in breast cancer.

Original languageEnglish (US)
Pages (from-to)3441-3445
Number of pages5
JournalCancer Research
Volume56
Issue number15
StatePublished - Aug 1 1996
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 20
Breast Neoplasms
Interphase
Fluorescence In Situ Hybridization
Oncogenes
Neoplasms
Cell Line
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tanner, M. M., Tirkkonen, M., Kallioniemi, A., Isola, J., Kuukasjärvi, T., Collins, C., ... Kallioniemi, O. P. (1996). Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. Cancer Research, 56(15), 3441-3445.

Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. / Tanner, Minna M.; Tirkkonen, Mika; Kallioniemi, Anne; Isola, Jorma; Kuukasjärvi, Tuula; Collins, Colin; Kowbel, David; Guan, Xin Yuan; Trent, Jeff; Gray, Joe; Meltzer, Paul; Kallioniemi, Olli P.

In: Cancer Research, Vol. 56, No. 15, 01.08.1996, p. 3441-3445.

Research output: Contribution to journalArticle

Tanner, MM, Tirkkonen, M, Kallioniemi, A, Isola, J, Kuukasjärvi, T, Collins, C, Kowbel, D, Guan, XY, Trent, J, Gray, J, Meltzer, P & Kallioniemi, OP 1996, 'Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer', Cancer Research, vol. 56, no. 15, pp. 3441-3445.
Tanner MM, Tirkkonen M, Kallioniemi A, Isola J, Kuukasjärvi T, Collins C et al. Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. Cancer Research. 1996 Aug 1;56(15):3441-3445.
Tanner, Minna M. ; Tirkkonen, Mika ; Kallioniemi, Anne ; Isola, Jorma ; Kuukasjärvi, Tuula ; Collins, Colin ; Kowbel, David ; Guan, Xin Yuan ; Trent, Jeff ; Gray, Joe ; Meltzer, Paul ; Kallioniemi, Olli P. / Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. In: Cancer Research. 1996 ; Vol. 56, No. 15. pp. 3441-3445.
@article{74a8c95ee90542e3b5dd9bdbee39e874,
title = "Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer",
abstract = "DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6{\%} of the cases), PTPN1 region 3 Mb proximal (6.2{\%}), and A1B3 region at 20q11 (6.2{\%}). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co- amplification in breast cancer.",
author = "Tanner, {Minna M.} and Mika Tirkkonen and Anne Kallioniemi and Jorma Isola and Tuula Kuukasj{\"a}rvi and Colin Collins and David Kowbel and Guan, {Xin Yuan} and Jeff Trent and Joe Gray and Paul Meltzer and Kallioniemi, {Olli P.}",
year = "1996",
month = "8",
day = "1",
language = "English (US)",
volume = "56",
pages = "3441--3445",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "15",

}

TY - JOUR

T1 - Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer

AU - Tanner, Minna M.

AU - Tirkkonen, Mika

AU - Kallioniemi, Anne

AU - Isola, Jorma

AU - Kuukasjärvi, Tuula

AU - Collins, Colin

AU - Kowbel, David

AU - Guan, Xin Yuan

AU - Trent, Jeff

AU - Gray, Joe

AU - Meltzer, Paul

AU - Kallioniemi, Olli P.

PY - 1996/8/1

Y1 - 1996/8/1

N2 - DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6% of the cases), PTPN1 region 3 Mb proximal (6.2%), and A1B3 region at 20q11 (6.2%). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co- amplification in breast cancer.

AB - DNA amplification at 20q13.2 is common in breast cancer, correlates with poor prognosis, and may reflect location of an important oncogene. Recently, other regions along 20q were also found to undergo amplification. Here, amplification levels and patterns of co-amplification were analyzed by interphase fluorescence in situ hybridization at 14 loci along 20q in 146 uncultured breast carcinomas and 14 cell lines. Three regions were independently amplified in uncultured tumors: RMC20C001 region at 20q13.2 (highly amplified in 9.6% of the cases), PTPN1 region 3 Mb proximal (6.2%), and A1B3 region at 20q11 (6.2%). Co-amplifications involving two or three of these regions were seen in 11 of the 19 highly amplified tumors. The results suggest that three distinct nonsyntenic regions along 20q may be important and that complex chromosomal rearrangements underlie their frequent co- amplification in breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=8944227502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8944227502&partnerID=8YFLogxK

M3 - Article

VL - 56

SP - 3441

EP - 3445

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 15

ER -