Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep

Lowell Davis, A. Yu, Alan (Roger) Hohimer, B. Jiang, D. Kimberly, C. Martin, G. Semenza

Research output: Contribution to journalArticle

Abstract

In chronic fetal anemia there is an increase in cardiac mass and cardiac output that is sufficient to maintain systemic tissue oxygenation. Cardiac output increases by 50%, myocardial blood flow increases five fold and cardiac hypertrophy occurs as the heart/body weight ratio increases 30%. To investigate the role of hypoxia inducible factor-1, an activator of vascular endothelial growth factor transcription in coronary vascular growth we measured VEGF, HIF-1 and capillary morphometry in fetal sheep at ∼130 d gestation made chronically anemic by daily isovolemic hemorrhage for 6 d. Compared to controls the hematocrit was reduced from 353±1.5 to 14.8±0.7%. Minimal capillary diameter increased in both the left (3.9±0.1 vs 5.5±0.5μm) and right ventricle (4.5±0.1 vs 7.1±0.4μm, means±se, n=5). The intercapillary distance, decreased in both the left (7.3±0.5 vs 5.5±0.4μm) and right ventricle (8.1±0.2 vs 5.5±0.4μm) and capillary density increased in the right ventricle (8.7±0.4 vs 10.4±0.6 103/mm2). Compared to controls, in extracts of anemic hearts, VEGF protein increased 4.5 fold (p

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

cardiac output
myocardium
Vascular Endothelial Growth Factor A
Heart Ventricles
Sheep
Myocardium
heart
sheep
Cardiac Output
vascular endothelial growth factors
morphometry
hypertrophy
blood vessels
anemia
blood flow
hematocrit
hemorrhage
Hypoxia-Inducible Factor 1
Oxygenation
transcription (genetics)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Davis, L., Yu, A., Hohimer, A. R., Jiang, B., Kimberly, D., Martin, C., & Semenza, G. (1997). Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep. FASEB Journal, 11(3).

Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep. / Davis, Lowell; Yu, A.; Hohimer, Alan (Roger); Jiang, B.; Kimberly, D.; Martin, C.; Semenza, G.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Davis, L, Yu, A, Hohimer, AR, Jiang, B, Kimberly, D, Martin, C & Semenza, G 1997, 'Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep', FASEB Journal, vol. 11, no. 3.
Davis, Lowell ; Yu, A. ; Hohimer, Alan (Roger) ; Jiang, B. ; Kimberly, D. ; Martin, C. ; Semenza, G. / Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
@article{e70e0b5136d8405e8d6bec653e0a3b4b,
title = "Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep",
abstract = "In chronic fetal anemia there is an increase in cardiac mass and cardiac output that is sufficient to maintain systemic tissue oxygenation. Cardiac output increases by 50{\%}, myocardial blood flow increases five fold and cardiac hypertrophy occurs as the heart/body weight ratio increases 30{\%}. To investigate the role of hypoxia inducible factor-1, an activator of vascular endothelial growth factor transcription in coronary vascular growth we measured VEGF, HIF-1 and capillary morphometry in fetal sheep at ∼130 d gestation made chronically anemic by daily isovolemic hemorrhage for 6 d. Compared to controls the hematocrit was reduced from 353±1.5 to 14.8±0.7{\%}. Minimal capillary diameter increased in both the left (3.9±0.1 vs 5.5±0.5μm) and right ventricle (4.5±0.1 vs 7.1±0.4μm, means±se, n=5). The intercapillary distance, decreased in both the left (7.3±0.5 vs 5.5±0.4μm) and right ventricle (8.1±0.2 vs 5.5±0.4μm) and capillary density increased in the right ventricle (8.7±0.4 vs 10.4±0.6 103/mm2). Compared to controls, in extracts of anemic hearts, VEGF protein increased 4.5 fold (p",
author = "Lowell Davis and A. Yu and Hohimer, {Alan (Roger)} and B. Jiang and D. Kimberly and C. Martin and G. Semenza",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Increased vascularization VEGF and HIF-1 expression in myocardium of chronically anemic fetal sheep

AU - Davis, Lowell

AU - Yu, A.

AU - Hohimer, Alan (Roger)

AU - Jiang, B.

AU - Kimberly, D.

AU - Martin, C.

AU - Semenza, G.

PY - 1997

Y1 - 1997

N2 - In chronic fetal anemia there is an increase in cardiac mass and cardiac output that is sufficient to maintain systemic tissue oxygenation. Cardiac output increases by 50%, myocardial blood flow increases five fold and cardiac hypertrophy occurs as the heart/body weight ratio increases 30%. To investigate the role of hypoxia inducible factor-1, an activator of vascular endothelial growth factor transcription in coronary vascular growth we measured VEGF, HIF-1 and capillary morphometry in fetal sheep at ∼130 d gestation made chronically anemic by daily isovolemic hemorrhage for 6 d. Compared to controls the hematocrit was reduced from 353±1.5 to 14.8±0.7%. Minimal capillary diameter increased in both the left (3.9±0.1 vs 5.5±0.5μm) and right ventricle (4.5±0.1 vs 7.1±0.4μm, means±se, n=5). The intercapillary distance, decreased in both the left (7.3±0.5 vs 5.5±0.4μm) and right ventricle (8.1±0.2 vs 5.5±0.4μm) and capillary density increased in the right ventricle (8.7±0.4 vs 10.4±0.6 103/mm2). Compared to controls, in extracts of anemic hearts, VEGF protein increased 4.5 fold (p

AB - In chronic fetal anemia there is an increase in cardiac mass and cardiac output that is sufficient to maintain systemic tissue oxygenation. Cardiac output increases by 50%, myocardial blood flow increases five fold and cardiac hypertrophy occurs as the heart/body weight ratio increases 30%. To investigate the role of hypoxia inducible factor-1, an activator of vascular endothelial growth factor transcription in coronary vascular growth we measured VEGF, HIF-1 and capillary morphometry in fetal sheep at ∼130 d gestation made chronically anemic by daily isovolemic hemorrhage for 6 d. Compared to controls the hematocrit was reduced from 353±1.5 to 14.8±0.7%. Minimal capillary diameter increased in both the left (3.9±0.1 vs 5.5±0.5μm) and right ventricle (4.5±0.1 vs 7.1±0.4μm, means±se, n=5). The intercapillary distance, decreased in both the left (7.3±0.5 vs 5.5±0.4μm) and right ventricle (8.1±0.2 vs 5.5±0.4μm) and capillary density increased in the right ventricle (8.7±0.4 vs 10.4±0.6 103/mm2). Compared to controls, in extracts of anemic hearts, VEGF protein increased 4.5 fold (p

UR - http://www.scopus.com/inward/record.url?scp=33750198701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750198701&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750198701

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -