Increased vascular resistance due to a reduction in red cell deformability in the isolated hind limb of swine

George Pantely, Lyle J. Swenson, Cherry H. Tamblyn, Geoffrey V F Seaman, Cheryl G. Anselone, W. Ben Johnson, J. David Bristow

    Research output: Contribution to journalArticle

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    Abstract

    This study investigated whether red cells with reduced deformability impeded flow through the microcirculation. Red cells were made less deformable in their normal biconcave disc shape by incubation with 2% formaldehyde (fRBCs). The blood supply to the right hind limb was isolated in 26 swine and the femoral artery was instrumented with two fine catheters, a flow probe, and an inflatable occluder. Flow was measured over a range of different perfusion pressures during adenosine-induced vasodilation under control conditions (C) and during an infusion of fRBCs at 1 ml/kg per minute (not to exceed 20 ml) into the femoral artery. At the same perfusion pressure (P), flow was significantly reduced 5 min after the fRBC infusion: Flow at P = 20 mm Hg, C = 41 ml/min vs fRBC = 10 ml/min; at P = 40, C = 160 ml/min vs fRBC = 79 ml/min; and at P = 60, C = 278 ml/min vs fRBC = 147 ml/min, with P <0.02 for all comparisons. Flow was still significantly reduced 15 min after the fRBCs, but by 30 min, it had returned to the control value. Chromium-51-labeling of red cells revealed that about one-third of fRBCs was trapped in the microcirculation compared to less than 3% of normal cells. This reduction in flow with fRBC infusion was not altered by α blockade, indicating that adrenergically mediated spasm was not responsible for the reduced flow. Aspirin 35 mg/kg iv completely prevented the reduction in flow despite an absence of change in the percentage of fRBCs trapped. Thus, red cells with reduced deformability infused into the circulation caused a significant, but transient, reduction in flow. The reduction in flow was not primarily related to entrapment of the abnormal RBCs, but may be mediated through platelet aggregation or release of potent vasoconstrictor substances from platelets or endothelial cells.

    Original languageEnglish (US)
    Pages (from-to)86-100
    Number of pages15
    JournalMicrovascular Research
    Volume35
    Issue number1
    DOIs
    StatePublished - 1988

    Fingerprint

    Formability
    Vascular Resistance
    Swine
    Extremities
    Cells
    Microcirculation
    Platelets
    Femoral Artery
    Perfusion
    Catheters
    Endothelial cells
    Vasoconstrictor Agents
    Chromium
    Pressure
    Adenosine
    Labeling
    Formaldehyde
    Aspirin
    Spasm
    Blood

    ASJC Scopus subject areas

    • Biochemistry
    • Cardiology and Cardiovascular Medicine

    Cite this

    Pantely, G., Swenson, L. J., Tamblyn, C. H., Seaman, G. V. F., Anselone, C. G., Johnson, W. B., & Bristow, J. D. (1988). Increased vascular resistance due to a reduction in red cell deformability in the isolated hind limb of swine. Microvascular Research, 35(1), 86-100. https://doi.org/10.1016/0026-2862(88)90052-0

    Increased vascular resistance due to a reduction in red cell deformability in the isolated hind limb of swine. / Pantely, George; Swenson, Lyle J.; Tamblyn, Cherry H.; Seaman, Geoffrey V F; Anselone, Cheryl G.; Johnson, W. Ben; Bristow, J. David.

    In: Microvascular Research, Vol. 35, No. 1, 1988, p. 86-100.

    Research output: Contribution to journalArticle

    Pantely, G, Swenson, LJ, Tamblyn, CH, Seaman, GVF, Anselone, CG, Johnson, WB & Bristow, JD 1988, 'Increased vascular resistance due to a reduction in red cell deformability in the isolated hind limb of swine', Microvascular Research, vol. 35, no. 1, pp. 86-100. https://doi.org/10.1016/0026-2862(88)90052-0
    Pantely, George ; Swenson, Lyle J. ; Tamblyn, Cherry H. ; Seaman, Geoffrey V F ; Anselone, Cheryl G. ; Johnson, W. Ben ; Bristow, J. David. / Increased vascular resistance due to a reduction in red cell deformability in the isolated hind limb of swine. In: Microvascular Research. 1988 ; Vol. 35, No. 1. pp. 86-100.
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    abstract = "This study investigated whether red cells with reduced deformability impeded flow through the microcirculation. Red cells were made less deformable in their normal biconcave disc shape by incubation with 2{\%} formaldehyde (fRBCs). The blood supply to the right hind limb was isolated in 26 swine and the femoral artery was instrumented with two fine catheters, a flow probe, and an inflatable occluder. Flow was measured over a range of different perfusion pressures during adenosine-induced vasodilation under control conditions (C) and during an infusion of fRBCs at 1 ml/kg per minute (not to exceed 20 ml) into the femoral artery. At the same perfusion pressure (P), flow was significantly reduced 5 min after the fRBC infusion: Flow at P = 20 mm Hg, C = 41 ml/min vs fRBC = 10 ml/min; at P = 40, C = 160 ml/min vs fRBC = 79 ml/min; and at P = 60, C = 278 ml/min vs fRBC = 147 ml/min, with P <0.02 for all comparisons. Flow was still significantly reduced 15 min after the fRBCs, but by 30 min, it had returned to the control value. Chromium-51-labeling of red cells revealed that about one-third of fRBCs was trapped in the microcirculation compared to less than 3{\%} of normal cells. This reduction in flow with fRBC infusion was not altered by α blockade, indicating that adrenergically mediated spasm was not responsible for the reduced flow. Aspirin 35 mg/kg iv completely prevented the reduction in flow despite an absence of change in the percentage of fRBCs trapped. Thus, red cells with reduced deformability infused into the circulation caused a significant, but transient, reduction in flow. The reduction in flow was not primarily related to entrapment of the abnormal RBCs, but may be mediated through platelet aggregation or release of potent vasoconstrictor substances from platelets or endothelial cells.",
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