Increased thromboxane production in recalcified challenged whole blood from copper-deficient rats

Kenneth G D Allen, Kirsten Lampi, Peter J. Bostwick, Melvin M. Mathias

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The influence of dietary copper deficiency on thrombin and collagen challenged whole blood thromboxane (TXA2) production was examined in rats. Citrated blood was collected from the abdominal aorta, recalcified to 5 mM Ca2+ and challenged with collagen or thrombin at 37° for 4 minutes. TX production was stopped with aspirin and TX measured by RIA. In two separate experiments dietary copper deficiency significantly increased TX production by 34% and 35% in collagen-challenged whole blood in comparison to Cu adequate control animals. TX production in response to a thrombin challenge also increased by 18% and 47%, but was significant in only one experiment. Platelet numbers were normal and unaffected by copper deficiency. We conclude that dietary copper deficiency enhances TX production in recalcified challenged whole blood and discuss the several possible mechanisms.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalNutrition Research
Volume11
Issue number1
DOIs
Publication statusPublished - 1991
Externally publishedYes

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Keywords

  • dietary copper deficiency
  • thromboxane production
  • whole blood

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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