Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus

David Calverley, Michele R. Hacker, Kimberly A. Loda, Elizabeth Brass, Thomas A. Buchanan, Denice D. Tsao-Wei, Susan Groshen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P <0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalBritish Journal of Haematology
Volume121
Issue number1
DOIs
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Fc Receptors
Diabetes Mellitus
Hypersensitivity
Collagen
Blood Platelets
Platelet Activation
Collagen Receptors
Immunoprecipitation
Tyrosine
Flow Cytometry
Thrombosis
Morbidity
Mortality

Keywords

  • Blood platelets
  • Cardiovascular disease
  • Collagen
  • Diabetes mellitus
  • Fc
  • Receptors

ASJC Scopus subject areas

  • Hematology

Cite this

Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus. / Calverley, David; Hacker, Michele R.; Loda, Kimberly A.; Brass, Elizabeth; Buchanan, Thomas A.; Tsao-Wei, Denice D.; Groshen, Susan.

In: British Journal of Haematology, Vol. 121, No. 1, 04.2003, p. 139-142.

Research output: Contribution to journalArticle

Calverley, David ; Hacker, Michele R. ; Loda, Kimberly A. ; Brass, Elizabeth ; Buchanan, Thomas A. ; Tsao-Wei, Denice D. ; Groshen, Susan. / Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus. In: British Journal of Haematology. 2003 ; Vol. 121, No. 1. pp. 139-142.
@article{46434e4562044bbdace021db280f2b4a,
title = "Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus",
abstract = "Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P <0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.",
keywords = "Blood platelets, Cardiovascular disease, Collagen, Diabetes mellitus, Fc, Receptors",
author = "David Calverley and Hacker, {Michele R.} and Loda, {Kimberly A.} and Elizabeth Brass and Buchanan, {Thomas A.} and Tsao-Wei, {Denice D.} and Susan Groshen",
year = "2003",
month = "4",
doi = "10.1046/j.1365-2141.2003.04233.x",
language = "English (US)",
volume = "121",
pages = "139--142",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus

AU - Calverley, David

AU - Hacker, Michele R.

AU - Loda, Kimberly A.

AU - Brass, Elizabeth

AU - Buchanan, Thomas A.

AU - Tsao-Wei, Denice D.

AU - Groshen, Susan

PY - 2003/4

Y1 - 2003/4

N2 - Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P <0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

AB - Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P <0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

KW - Blood platelets

KW - Cardiovascular disease

KW - Collagen

KW - Diabetes mellitus

KW - Fc

KW - Receptors

UR - http://www.scopus.com/inward/record.url?scp=0037392234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037392234&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2141.2003.04233.x

DO - 10.1046/j.1365-2141.2003.04233.x

M3 - Article

C2 - 12670344

AN - SCOPUS:0037392234

VL - 121

SP - 139

EP - 142

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 1

ER -