Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats

Vemuganti L. Raghavendra Rao, Mustafa K. Başkaya, A. Muralikrishna Rao, Aclan Doǧan, Robert J. Dempsey

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

There is increasing evidence that the elevated levels of polyamines play an important role in the secondary injury following traumatic brain injury (TBI). Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine biosynthesis. Presently, we measured the ODC protein levels by Western blot analysis in the cerebral cortex of rats sacrificed at 2 h, 6 h, 24 h, 72 h and 168 h after controlled cortical impact injury. TBI resulted in a significant increase in ODC protein levels (2.5 to 5.5 fold, P < 0.05) and enzyme activity (13 to 21 fold, p < 0.01) between 2 and 6 h after the injury. ODC protein levels and enzyme activity returned to normal, control levels by 72 h after the injury. Increased ODC protein and enzyme activity could contribute to vasogenic edema and the pathogenesis of neuronal dysfunction after TBI by stimulating the formation of polyamines.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalBrain research
Volume783
Issue number1
DOIs
StatePublished - Feb 2 1998
Externally publishedYes

Keywords

  • Ornithine decarboxylase
  • Polyamine
  • Traumatic brain injury
  • Western blotting

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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