Increased leukocyte histamine release with elevated cyclic AMP-phosphodiesterase activity in atopic dermatitis

James M. Butler, Sai C. Chan, Seth Stevens, Jon Hanifin

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Maximal leukocyte histamine release in response to concanavalin A was significantly higher in a group of 16 adult patients with moderate to severe atopic dermatitis when compared to 13 adult nonatopic, normal subjects. In a further four atopic dermatitis patients, histamine release was similar to that in the normal group suggesting the existence of "high-releaser" and "low-releaser" subsets within the atopic dermatitis group. Leukocyte cAMP phosphodiesterase activity was significantly higher in the high-releaser group than in the low-releaser and normal groups. High and low histamine release responses showed strong correlations with high and low phosphodiesterase activities. Pretreatment with the experimental cAMP phosphodiesterase inhibitor Ro-20-1724 in high releasers reduced the histamine release to normal levels. These findings suggest that increased histamine "releasability" in atopic dermatitis is related to abnormalities in cyclic nucleotide regulation. Basophil percentages within the leukocyte preparation and the histamine content per basophil were not significantly different between the atopics and normals. Histamine release did not correlate significantly with serum IgE levels.

Original languageEnglish (US)
Pages (from-to)490-497
Number of pages8
JournalThe Journal of allergy and clinical immunology
Volume71
Issue number5
DOIs
StatePublished - 1983

Fingerprint

Histamine Release
Phosphoric Diester Hydrolases
Atopic Dermatitis
Cyclic AMP
Leukocytes
Basophils
Histamine
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
Phosphodiesterase Inhibitors
Cyclic Nucleotides
Concanavalin A
Immunoglobulin E
Serum

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Increased leukocyte histamine release with elevated cyclic AMP-phosphodiesterase activity in atopic dermatitis. / Butler, James M.; Chan, Sai C.; Stevens, Seth; Hanifin, Jon.

In: The Journal of allergy and clinical immunology, Vol. 71, No. 5, 1983, p. 490-497.

Research output: Contribution to journalArticle

@article{03f771d1f8214048b049237411d5e069,
title = "Increased leukocyte histamine release with elevated cyclic AMP-phosphodiesterase activity in atopic dermatitis",
abstract = "Maximal leukocyte histamine release in response to concanavalin A was significantly higher in a group of 16 adult patients with moderate to severe atopic dermatitis when compared to 13 adult nonatopic, normal subjects. In a further four atopic dermatitis patients, histamine release was similar to that in the normal group suggesting the existence of {"}high-releaser{"} and {"}low-releaser{"} subsets within the atopic dermatitis group. Leukocyte cAMP phosphodiesterase activity was significantly higher in the high-releaser group than in the low-releaser and normal groups. High and low histamine release responses showed strong correlations with high and low phosphodiesterase activities. Pretreatment with the experimental cAMP phosphodiesterase inhibitor Ro-20-1724 in high releasers reduced the histamine release to normal levels. These findings suggest that increased histamine {"}releasability{"} in atopic dermatitis is related to abnormalities in cyclic nucleotide regulation. Basophil percentages within the leukocyte preparation and the histamine content per basophil were not significantly different between the atopics and normals. Histamine release did not correlate significantly with serum IgE levels.",
author = "Butler, {James M.} and Chan, {Sai C.} and Seth Stevens and Jon Hanifin",
year = "1983",
doi = "10.1016/0091-6749(83)90467-0",
language = "English (US)",
volume = "71",
pages = "490--497",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Increased leukocyte histamine release with elevated cyclic AMP-phosphodiesterase activity in atopic dermatitis

AU - Butler, James M.

AU - Chan, Sai C.

AU - Stevens, Seth

AU - Hanifin, Jon

PY - 1983

Y1 - 1983

N2 - Maximal leukocyte histamine release in response to concanavalin A was significantly higher in a group of 16 adult patients with moderate to severe atopic dermatitis when compared to 13 adult nonatopic, normal subjects. In a further four atopic dermatitis patients, histamine release was similar to that in the normal group suggesting the existence of "high-releaser" and "low-releaser" subsets within the atopic dermatitis group. Leukocyte cAMP phosphodiesterase activity was significantly higher in the high-releaser group than in the low-releaser and normal groups. High and low histamine release responses showed strong correlations with high and low phosphodiesterase activities. Pretreatment with the experimental cAMP phosphodiesterase inhibitor Ro-20-1724 in high releasers reduced the histamine release to normal levels. These findings suggest that increased histamine "releasability" in atopic dermatitis is related to abnormalities in cyclic nucleotide regulation. Basophil percentages within the leukocyte preparation and the histamine content per basophil were not significantly different between the atopics and normals. Histamine release did not correlate significantly with serum IgE levels.

AB - Maximal leukocyte histamine release in response to concanavalin A was significantly higher in a group of 16 adult patients with moderate to severe atopic dermatitis when compared to 13 adult nonatopic, normal subjects. In a further four atopic dermatitis patients, histamine release was similar to that in the normal group suggesting the existence of "high-releaser" and "low-releaser" subsets within the atopic dermatitis group. Leukocyte cAMP phosphodiesterase activity was significantly higher in the high-releaser group than in the low-releaser and normal groups. High and low histamine release responses showed strong correlations with high and low phosphodiesterase activities. Pretreatment with the experimental cAMP phosphodiesterase inhibitor Ro-20-1724 in high releasers reduced the histamine release to normal levels. These findings suggest that increased histamine "releasability" in atopic dermatitis is related to abnormalities in cyclic nucleotide regulation. Basophil percentages within the leukocyte preparation and the histamine content per basophil were not significantly different between the atopics and normals. Histamine release did not correlate significantly with serum IgE levels.

UR - http://www.scopus.com/inward/record.url?scp=0020519162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020519162&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(83)90467-0

DO - 10.1016/0091-6749(83)90467-0

M3 - Article

C2 - 6188771

AN - SCOPUS:0020519162

VL - 71

SP - 490

EP - 497

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 5

ER -