TY - JOUR
T1 - Increased interleukin 6 production by T cells derived from patients with atopic dermatitis
AU - Toshitani, Akito
AU - Ansel, John C.
AU - Chan, Sai C.
AU - Li, Shi Hua
AU - Hanifin, Jon M.
PY - 1993/3
Y1 - 1993/3
N2 - Many immunologic aspects of atopic dermatitis have been studied, but basic pathobiologic mechanisms of this disease remain unknown. In this study, we measured the production of interleukin-6 (IL-6) by peripheral blood T cells and monocytes from patients with atopic dermatitis in comparison to normal control subjects and patients with chronic psoriasis. We found that peripheral blood T cells isolated from patients with atopic dermatitis produced significantly higher levels of IL-6 (36.1 ± 5.1 units/ml, n = 22) than T cells derived from either normal subjects (12.6 ± 1.9 units/ml, n = 22) or patients with chronic psoriasis (26.7 ± 4.1 units/ml, n = 7). T-cell activation was also measured in the patients with atopic dermatitis by soluble serum IL-2 receptor levels and were found to be significantly higher (623.7 ± 8.1 units/ml, n = 8) than normal subjects (357.2 ± 26.0 units/ml, n = 8). In contrast to the increased production of IL-6 by T cells in atopic dermatitis, there was no significant difference in the IL-6 production by peripheral blood monocytes derived from patients with atopic dermatitis compared to normal subjects. Thus, peripheral blood T cells derived from patients with AD spontaneously produce increased amounts of IL-6 compared to T cells from normal subjects, which may reflect the increased activation state of T cells in atopic dermatitis. These data support the concept that activated T cells or subsets of T cells may be important effector cells in mediating inflammatory activity in atopic disease.
AB - Many immunologic aspects of atopic dermatitis have been studied, but basic pathobiologic mechanisms of this disease remain unknown. In this study, we measured the production of interleukin-6 (IL-6) by peripheral blood T cells and monocytes from patients with atopic dermatitis in comparison to normal control subjects and patients with chronic psoriasis. We found that peripheral blood T cells isolated from patients with atopic dermatitis produced significantly higher levels of IL-6 (36.1 ± 5.1 units/ml, n = 22) than T cells derived from either normal subjects (12.6 ± 1.9 units/ml, n = 22) or patients with chronic psoriasis (26.7 ± 4.1 units/ml, n = 7). T-cell activation was also measured in the patients with atopic dermatitis by soluble serum IL-2 receptor levels and were found to be significantly higher (623.7 ± 8.1 units/ml, n = 8) than normal subjects (357.2 ± 26.0 units/ml, n = 8). In contrast to the increased production of IL-6 by T cells in atopic dermatitis, there was no significant difference in the IL-6 production by peripheral blood monocytes derived from patients with atopic dermatitis compared to normal subjects. Thus, peripheral blood T cells derived from patients with AD spontaneously produce increased amounts of IL-6 compared to T cells from normal subjects, which may reflect the increased activation state of T cells in atopic dermatitis. These data support the concept that activated T cells or subsets of T cells may be important effector cells in mediating inflammatory activity in atopic disease.
UR - http://www.scopus.com/inward/record.url?scp=0027414362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027414362&partnerID=8YFLogxK
U2 - 10.1111/1523-1747.ep12469875
DO - 10.1111/1523-1747.ep12469875
M3 - Article
C2 - 8440909
AN - SCOPUS:0027414362
SN - 0022-202X
VL - 100
SP - 299
EP - 304
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -