Increased Copy Number at 20ql3 in Breast Cancer: Defining the Critical Region and Exclusion of Candidate Genes1

Minna M. Tanner, Mika Tirkkonen, Anne Kallioniemi, Colin Collins, Trond Stokke, Ritva Karhu, Dave Kowbel, Feridan Shadravan, Mary Hintz, Wen Lin Kuo, Frederic M. Waldman, Jorma J. Isola, Joe W. Gray, Olli P. Kallioniemi

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Abstract

Studies by comparative genomic hybridization have indicated that a major new locus for DNA amplification in breast cancer is 20ql3 and suggested that this genetic event is associated with aggressive clinical behavior. We used interphase fluorescence in situ hybridization with anonymous cosmid probes and gene-specific P1 clones to determine the minimal common region of increased copy number and to study involvement of known genes at 20ql3. Based on high-level copy number increases (3 to 10-fold) found with one or more probes in 5 of 14 (35%) breast cancer cell lines and in 3 of 36 (8%) primary tumors, the critical region was narrowed to ~1.5 megabases at 20ql3.2 defined by fractional length pter values 0.81-0.84. Previously known genes were excluded as candidates, implying that this chromosomal region harbors a novel oncogene that contributes to the malignant progression of breast cancer.

Original languageEnglish (US)
Pages (from-to)4257-4260
Number of pages4
JournalCancer Research
Volume54
Issue number16
StatePublished - Aug 1994

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Tanner, M. M., Tirkkonen, M., Kallioniemi, A., Collins, C., Stokke, T., Karhu, R., Kowbel, D., Shadravan, F., Hintz, M., Kuo, W. L., Waldman, F. M., Isola, J. J., Gray, J. W., & Kallioniemi, O. P. (1994). Increased Copy Number at 20ql3 in Breast Cancer: Defining the Critical Region and Exclusion of Candidate Genes1. Cancer Research, 54(16), 4257-4260.