Increased copy number at 20q13 in breast cancer

Defining the critical region and exclusion of candidate genes

Minna M. Tanner, Mika Tirkkonen, Anne Kallioniemi, Colin Collins, Trond Stokke, Ritva Karhu, Dave Kowbel, Feridan Shadravan, Mary Hintz, Wen Lin Kuo, Frederic M. Waldman, Jorma J. Isola, Joe Gray, Olli P. Kallioniemi

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Studies by comparative genomic hybridization have indicated that a major new locus for DNA amplification in breast cancer is 20q13 and suggested that this genetic event is associated with aggressive clinical behavior. We used interphase fluorescence in situ hybridization with anonymous cosmid probes and gene-specific P1 clones to determine the minimal common region of increased copy number and to study involvement of known genes at 20q13. Based on high-level copy number increases (3 to 10-fold) found with one or more probes in 5 of 14 (35%) breast cancer cell lines and in 3 of 36 (8%) primary tumors, the critical region was narrowed to ~1.5 megabases at 20q13.2 defined by fractional length pter values 0.81-0.84. Previously known genes were excluded as candidates, implying that this chromosomal region harbors a novel oncogene that contributes to the malignant progression of breast cancer.

Original languageEnglish (US)
Pages (from-to)4257-4260
Number of pages4
JournalCancer Research
Volume54
Issue number16
StatePublished - Aug 15 1994
Externally publishedYes

Fingerprint

Breast Neoplasms
Genes
Cosmids
Comparative Genomic Hybridization
Interphase
Fluorescence In Situ Hybridization
Oncogenes
Clone Cells
Cell Line
DNA
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tanner, M. M., Tirkkonen, M., Kallioniemi, A., Collins, C., Stokke, T., Karhu, R., ... Kallioniemi, O. P. (1994). Increased copy number at 20q13 in breast cancer: Defining the critical region and exclusion of candidate genes. Cancer Research, 54(16), 4257-4260.

Increased copy number at 20q13 in breast cancer : Defining the critical region and exclusion of candidate genes. / Tanner, Minna M.; Tirkkonen, Mika; Kallioniemi, Anne; Collins, Colin; Stokke, Trond; Karhu, Ritva; Kowbel, Dave; Shadravan, Feridan; Hintz, Mary; Kuo, Wen Lin; Waldman, Frederic M.; Isola, Jorma J.; Gray, Joe; Kallioniemi, Olli P.

In: Cancer Research, Vol. 54, No. 16, 15.08.1994, p. 4257-4260.

Research output: Contribution to journalArticle

Tanner, MM, Tirkkonen, M, Kallioniemi, A, Collins, C, Stokke, T, Karhu, R, Kowbel, D, Shadravan, F, Hintz, M, Kuo, WL, Waldman, FM, Isola, JJ, Gray, J & Kallioniemi, OP 1994, 'Increased copy number at 20q13 in breast cancer: Defining the critical region and exclusion of candidate genes', Cancer Research, vol. 54, no. 16, pp. 4257-4260.
Tanner MM, Tirkkonen M, Kallioniemi A, Collins C, Stokke T, Karhu R et al. Increased copy number at 20q13 in breast cancer: Defining the critical region and exclusion of candidate genes. Cancer Research. 1994 Aug 15;54(16):4257-4260.
Tanner, Minna M. ; Tirkkonen, Mika ; Kallioniemi, Anne ; Collins, Colin ; Stokke, Trond ; Karhu, Ritva ; Kowbel, Dave ; Shadravan, Feridan ; Hintz, Mary ; Kuo, Wen Lin ; Waldman, Frederic M. ; Isola, Jorma J. ; Gray, Joe ; Kallioniemi, Olli P. / Increased copy number at 20q13 in breast cancer : Defining the critical region and exclusion of candidate genes. In: Cancer Research. 1994 ; Vol. 54, No. 16. pp. 4257-4260.
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