Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages

Sergio Fazio, Vladimir R. Babaev, Alisa B. Murray, Alyssa H. Hasty, Kathy J. Carter, Linda A. Gleaves, James B. Atkinson, Macrae F. Linton

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

Macrophage-derived foam cells express apolipoprotein E (apoE) abundantly in atheroscleratic lesions. To examine the physiologic role of apoe secretion by the macrophage in atherogenesis, bone marrow transplantation was used to reconstitute C57bl/6 mice with macrophages that were either null or wild type for the apoE gene. After 13 weeks on an atherogenic diet, C57bl/6 mice reconstituted with apoE null marrow developed 10-fold more atherosclerosis than controls in the absence of significant differences in serum cholesterol levels or lipoprotein profiles. ApoE expression was absent in the macrophage- derived foam cells of C57bl/6 mice reconstituted with apoE null marrow. Thus, lack of apoE expression by the macrophage promotes foam cell formation. These data support a protective role for apoE expression by the macrophage in early atherogenesis.

Original languageEnglish (US)
Pages (from-to)4647-4652
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number9
DOIs
StatePublished - Apr 29 1997
Externally publishedYes

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Apolipoproteins E
Atherosclerosis
Macrophages
Foam Cells
Bone Marrow
Atherogenic Diet
Bone Marrow Transplantation
Lipoproteins
Cholesterol
Serum
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages. / Fazio, Sergio; Babaev, Vladimir R.; Murray, Alisa B.; Hasty, Alyssa H.; Carter, Kathy J.; Gleaves, Linda A.; Atkinson, James B.; Linton, Macrae F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 9, 29.04.1997, p. 4647-4652.

Research output: Contribution to journalArticle

Fazio, Sergio ; Babaev, Vladimir R. ; Murray, Alisa B. ; Hasty, Alyssa H. ; Carter, Kathy J. ; Gleaves, Linda A. ; Atkinson, James B. ; Linton, Macrae F. / Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 9. pp. 4647-4652.
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AU - Atkinson, James B.

AU - Linton, Macrae F.

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