Increased amounts of the Aspergillus metabolite D-mannitol in tissue and serum of rats with experimental aspergillosis

Brian Wong, K. L. Brauer, R. R. Tsai, K. Jayasimhulu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Several Aspergillus species produce large amounts of the hexitol D-mannitol in vitro, but it is not known whether these species also produce D-mannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 x 106 preincubated conidia of Aspergillus fumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be D-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more D-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P <.01 at 12, 24, and 36 h) and higher serum D-mannitol concentrations and serum D-mannitol/creatinine ratios after 36 h (P <.05) than did uninfected controls. These results indicate that A. fumigatus can produce and release sufficient D-mannitol in the tissues of infected animals to raise serum D-mannitol levels. Thus, D-mannitol is a potential diagnostic marker for aspergillosis.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalJournal of Infectious Diseases
Volume160
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Aspergillosis
Mannitol
Aspergillus
Serum
Aspergillus fumigatus
Gas Chromatography
Kidney
Lung
Fungal Spores
Liver
Cortisone
Cyclophosphamide
Gas Chromatography-Mass Spectrometry
Liver Diseases
Creatinine
Spleen

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Increased amounts of the Aspergillus metabolite D-mannitol in tissue and serum of rats with experimental aspergillosis. / Wong, Brian; Brauer, K. L.; Tsai, R. R.; Jayasimhulu, K.

In: Journal of Infectious Diseases, Vol. 160, No. 1, 1989, p. 95-103.

Research output: Contribution to journalArticle

Wong, Brian ; Brauer, K. L. ; Tsai, R. R. ; Jayasimhulu, K. / Increased amounts of the Aspergillus metabolite D-mannitol in tissue and serum of rats with experimental aspergillosis. In: Journal of Infectious Diseases. 1989 ; Vol. 160, No. 1. pp. 95-103.
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