Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

M. Riepe, A. Ludolph, M. Seelig, Peter Spencer, A. C. Ludolph

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

Original languageEnglish (US)
Pages (from-to)2130-2132
Number of pages3
JournalNeuroReport
Volume5
Issue number16
StatePublished - 1994

Fingerprint

Excitatory Amino Acid Antagonists
6-Cyano-7-nitroquinoxaline-2,3-dione
Dizocilpine Maleate
Adenosine Triphosphate
Succinate Dehydrogenase
Neuroprotective Agents
Neuroprotection
3-nitropropionic acid

Keywords

  • 3-NPA
  • ATP
  • Excitoxicity
  • Glutamate
  • Glutamate antagonist
  • Neurodegeneration
  • SDH

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Riepe, M., Ludolph, A., Seelig, M., Spencer, P., & Ludolph, A. C. (1994). Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection. NeuroReport, 5(16), 2130-2132.

Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection. / Riepe, M.; Ludolph, A.; Seelig, M.; Spencer, Peter; Ludolph, A. C.

In: NeuroReport, Vol. 5, No. 16, 1994, p. 2130-2132.

Research output: Contribution to journalArticle

Riepe, M, Ludolph, A, Seelig, M, Spencer, P & Ludolph, AC 1994, 'Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection', NeuroReport, vol. 5, no. 16, pp. 2130-2132.
Riepe, M. ; Ludolph, A. ; Seelig, M. ; Spencer, Peter ; Ludolph, A. C. / Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection. In: NeuroReport. 1994 ; Vol. 5, No. 16. pp. 2130-2132.
@article{e4a23e9c30c549929ec3cdb8a4b9a07b,
title = "Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection",
abstract = "Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.",
keywords = "3-NPA, ATP, Excitoxicity, Glutamate, Glutamate antagonist, Neurodegeneration, SDH",
author = "M. Riepe and A. Ludolph and M. Seelig and Peter Spencer and Ludolph, {A. C.}",
year = "1994",
language = "English (US)",
volume = "5",
pages = "2130--2132",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "16",

}

TY - JOUR

T1 - Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

AU - Riepe, M.

AU - Ludolph, A.

AU - Seelig, M.

AU - Spencer, Peter

AU - Ludolph, A. C.

PY - 1994

Y1 - 1994

N2 - Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

AB - Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

KW - 3-NPA

KW - ATP

KW - Excitoxicity

KW - Glutamate

KW - Glutamate antagonist

KW - Neurodegeneration

KW - SDH

UR - http://www.scopus.com/inward/record.url?scp=0028151690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028151690&partnerID=8YFLogxK

M3 - Article

C2 - 7865761

AN - SCOPUS:0028151690

VL - 5

SP - 2130

EP - 2132

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 16

ER -