Incorporation of vitronectin into fibrin clots. Evidence for a binding interaction between vitronectin and γA/γ′ fibrinogen

Thomas J. Podor, Stephanie Campbell, Paul Chindemi, Denise M. Foulon, David Farrell, Philip D. Walton, Jeffrey I. Weitz, Cynthia B. Peterson

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Vitronectin is an abundant plasma protein that regulates coagulation, fibrinolysis, complement activation, and cell adhesion. Recently, we demonstrated that plasma vitronectin inhibits fibrinolysis by mediating the interaction of type 1 plasminogen activator inhibitor with fibrin (Podor, T. J., Peterson, C. B., Lawrence, D. A., Stefansson, S., Shaughnessy, S. G., Foulon, D. M., Butcher, M., and Weitz, J. I. (2000) J. Biol. Chem. 275, 19788-19794). The current studies were undertaken to further examine the interactions between vitronectin and fibrin(ogen). Comparison of vitronectin levels in plasma with those in serum indicates that ∼20% of plasma vitronectin is incorporated into the clot. When the time course of biotinylated-vitronectin incorporation into clots formed from 125I-fibrinogen is monitored, vitronectin incorporation into the clot parallels that of fibrinogen in the absence or presence of activated factor XIII. Vitronectin binds specifically to fibrin matrices with an estimated Kd of ∼0.6 μM. Additional vitronectin subunits are assembled on fibrin-bound vitronectin multimers through self-association. Confocal microscopy of fibrin clots reveals the globular vitronectin aggregates anchored at intervals along the fibrin fibrils. This periodicity raised the possibility that vitronectin interacts with the γA/γ variant of fibrin(ogen) that represents about 10% of total fibrinogen. In support of this concept, the vitronectin which contaminates fibrinogen preparations co-purifies with the γA/γ fibrinogen fraction, and clots formed from γA/γ fibrinogen preferentially bind vitronectin. These studies reveal that vitronectin associates with fibrin during coagulation, and may thereby modulate hemostasis and inflammation.

Original languageEnglish (US)
Pages (from-to)7520-7528
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number9
DOIs
StatePublished - Mar 1 2002
Externally publishedYes

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Vitronectin
Fibrin
Fibrinogen
Fibrinolysis
Coagulation
Plasmas
Factor XIIIa
Confocal microscopy
Complement Activation
Plasminogen Activator Inhibitor 1
Cell adhesion
Beam plasma interactions
Periodicity
Hemostasis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Incorporation of vitronectin into fibrin clots. Evidence for a binding interaction between vitronectin and γA/γ′ fibrinogen. / Podor, Thomas J.; Campbell, Stephanie; Chindemi, Paul; Foulon, Denise M.; Farrell, David; Walton, Philip D.; Weitz, Jeffrey I.; Peterson, Cynthia B.

In: Journal of Biological Chemistry, Vol. 277, No. 9, 01.03.2002, p. 7520-7528.

Research output: Contribution to journalArticle

Podor, Thomas J. ; Campbell, Stephanie ; Chindemi, Paul ; Foulon, Denise M. ; Farrell, David ; Walton, Philip D. ; Weitz, Jeffrey I. ; Peterson, Cynthia B. / Incorporation of vitronectin into fibrin clots. Evidence for a binding interaction between vitronectin and γA/γ′ fibrinogen. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 9. pp. 7520-7528.
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abstract = "Vitronectin is an abundant plasma protein that regulates coagulation, fibrinolysis, complement activation, and cell adhesion. Recently, we demonstrated that plasma vitronectin inhibits fibrinolysis by mediating the interaction of type 1 plasminogen activator inhibitor with fibrin (Podor, T. J., Peterson, C. B., Lawrence, D. A., Stefansson, S., Shaughnessy, S. G., Foulon, D. M., Butcher, M., and Weitz, J. I. (2000) J. Biol. Chem. 275, 19788-19794). The current studies were undertaken to further examine the interactions between vitronectin and fibrin(ogen). Comparison of vitronectin levels in plasma with those in serum indicates that ∼20{\%} of plasma vitronectin is incorporated into the clot. When the time course of biotinylated-vitronectin incorporation into clots formed from 125I-fibrinogen is monitored, vitronectin incorporation into the clot parallels that of fibrinogen in the absence or presence of activated factor XIII. Vitronectin binds specifically to fibrin matrices with an estimated Kd of ∼0.6 μM. Additional vitronectin subunits are assembled on fibrin-bound vitronectin multimers through self-association. Confocal microscopy of fibrin clots reveals the globular vitronectin aggregates anchored at intervals along the fibrin fibrils. This periodicity raised the possibility that vitronectin interacts with the γA/γ′ variant of fibrin(ogen) that represents about 10{\%} of total fibrinogen. In support of this concept, the vitronectin which contaminates fibrinogen preparations co-purifies with the γA/γ′ fibrinogen fraction, and clots formed from γA/γ′ fibrinogen preferentially bind vitronectin. These studies reveal that vitronectin associates with fibrin during coagulation, and may thereby modulate hemostasis and inflammation.",
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AU - Podor, Thomas J.

AU - Campbell, Stephanie

AU - Chindemi, Paul

AU - Foulon, Denise M.

AU - Farrell, David

AU - Walton, Philip D.

AU - Weitz, Jeffrey I.

AU - Peterson, Cynthia B.

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AB - Vitronectin is an abundant plasma protein that regulates coagulation, fibrinolysis, complement activation, and cell adhesion. Recently, we demonstrated that plasma vitronectin inhibits fibrinolysis by mediating the interaction of type 1 plasminogen activator inhibitor with fibrin (Podor, T. J., Peterson, C. B., Lawrence, D. A., Stefansson, S., Shaughnessy, S. G., Foulon, D. M., Butcher, M., and Weitz, J. I. (2000) J. Biol. Chem. 275, 19788-19794). The current studies were undertaken to further examine the interactions between vitronectin and fibrin(ogen). Comparison of vitronectin levels in plasma with those in serum indicates that ∼20% of plasma vitronectin is incorporated into the clot. When the time course of biotinylated-vitronectin incorporation into clots formed from 125I-fibrinogen is monitored, vitronectin incorporation into the clot parallels that of fibrinogen in the absence or presence of activated factor XIII. Vitronectin binds specifically to fibrin matrices with an estimated Kd of ∼0.6 μM. Additional vitronectin subunits are assembled on fibrin-bound vitronectin multimers through self-association. Confocal microscopy of fibrin clots reveals the globular vitronectin aggregates anchored at intervals along the fibrin fibrils. This periodicity raised the possibility that vitronectin interacts with the γA/γ′ variant of fibrin(ogen) that represents about 10% of total fibrinogen. In support of this concept, the vitronectin which contaminates fibrinogen preparations co-purifies with the γA/γ′ fibrinogen fraction, and clots formed from γA/γ′ fibrinogen preferentially bind vitronectin. These studies reveal that vitronectin associates with fibrin during coagulation, and may thereby modulate hemostasis and inflammation.

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