Incorporation of Non-Canonical Amino Acids

Lilia Leisle, Francis Valiyaveetil, Ryan A. Mehl, Christopher A. Ahern

Research output: Contribution to journalReview article

10 Scopus citations

Abstract

In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.

Original languageEnglish (US)
Pages (from-to)119-151
Number of pages33
JournalAdvances in experimental medicine and biology
Volume869
DOIs
StatePublished - 2015

Keywords

  • Evolved tRNA synthetase pairs
  • Intein ligation
  • Nonsense suppression
  • Protein semi-synthesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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