Incorporation of non-canonical amino acids

Lilia Leisle, Francis Valiyaveetil, Ryan A. Mehl, Christopher A. Ahern

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.

Original languageEnglish (US)
Title of host publicationNovel Chemical Tools to Study Ion Channel Biology
PublisherSpringer New York
Pages119-151
Number of pages33
ISBN (Print)9781493928453, 9781493928446
DOIs
StatePublished - Sep 17 2015

Fingerprint

Membrane Proteins
Amino Acyl-tRNA Synthetases
Amino Acids
Xenopus laevis
Transfer RNA
Ion Channels
Oocytes
Ligation
Proteins
Chemical analysis

Keywords

  • Evolved tRNA synthetase pairs
  • Intein ligation
  • Nonsense suppression
  • Protein semi-synthesis

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Leisle, L., Valiyaveetil, F., Mehl, R. A., & Ahern, C. A. (2015). Incorporation of non-canonical amino acids. In Novel Chemical Tools to Study Ion Channel Biology (pp. 119-151). Springer New York. https://doi.org/10.1007/978-1-4939-2845-3_7

Incorporation of non-canonical amino acids. / Leisle, Lilia; Valiyaveetil, Francis; Mehl, Ryan A.; Ahern, Christopher A.

Novel Chemical Tools to Study Ion Channel Biology. Springer New York, 2015. p. 119-151.

Research output: Chapter in Book/Report/Conference proceedingChapter

Leisle, L, Valiyaveetil, F, Mehl, RA & Ahern, CA 2015, Incorporation of non-canonical amino acids. in Novel Chemical Tools to Study Ion Channel Biology. Springer New York, pp. 119-151. https://doi.org/10.1007/978-1-4939-2845-3_7
Leisle L, Valiyaveetil F, Mehl RA, Ahern CA. Incorporation of non-canonical amino acids. In Novel Chemical Tools to Study Ion Channel Biology. Springer New York. 2015. p. 119-151 https://doi.org/10.1007/978-1-4939-2845-3_7
Leisle, Lilia ; Valiyaveetil, Francis ; Mehl, Ryan A. ; Ahern, Christopher A. / Incorporation of non-canonical amino acids. Novel Chemical Tools to Study Ion Channel Biology. Springer New York, 2015. pp. 119-151
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