TY - JOUR
T1 - Incorporation of Non-Canonical Amino Acids
AU - Leisle, Lilia
AU - Valiyaveetil, Francis
AU - Mehl, Ryan A.
AU - Ahern, Christopher A.
N1 - Copyright:
Copyright 2016 Medline is the source for the citation and abstract of this record.
PY - 2015
Y1 - 2015
N2 - In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.
AB - In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.
KW - Evolved tRNA synthetase pairs
KW - Intein ligation
KW - Nonsense suppression
KW - Protein semi-synthesis
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U2 - 10.1007/978-1-4939-2845-3_7
DO - 10.1007/978-1-4939-2845-3_7
M3 - Review article
C2 - 26381943
AN - SCOPUS:84955151935
VL - 869
SP - 119
EP - 151
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -