TY - JOUR
T1 - Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence
AU - André, Carolina Bosso
AU - Rosalen, Pedro Luiz
AU - Giannini, Marcelo
AU - Bueno-Silva, Bruno
AU - Pfeifer, Carmem Silvia
AU - Ferracane, Jack Liborio
N1 - Funding Information:
This research was supported by NIH/ NIDCR K02 DE025280 and Brazilian Financial Agencies: CNPq: 140698/2013-2, 310522/2015-3, and 150813/2017-1, FAPESP: 2013/22823-9 and 2014/15543-0, and CAPES 001. The authors declare no conflict of interest. The authors would like to thank Dr. Kristen Lampi and Satin Salehi (in memoriam) for assistance with the bacterial experiments and shared knowledge.
Publisher Copyright:
© 2020 The Academy of Dental Materials
PY - 2021/4
Y1 - 2021/4
N2 - Objectives: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
AB - Objectives: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
KW - Apigenin
KW - Dental caries
KW - Resin cement
KW - Resin composite
KW - Streptococcus mutans
KW - tt-Farnesol
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U2 - 10.1016/j.dental.2020.12.005
DO - 10.1016/j.dental.2020.12.005
M3 - Article
C2 - 33422299
AN - SCOPUS:85099146801
SN - 0109-5641
VL - 37
SP - e201-e212
JO - Dental Materials
JF - Dental Materials
IS - 4
ER -