Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence

Carolina Bosso André, Pedro Luiz Rosalen, Marcelo Giannini, Bruno Bueno-Silva, Carmem Silvia Pfeifer, Jack Liborio Ferracane

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Objectives: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.

Original languageEnglish (US)
Pages (from-to)e201-e212
JournalDental Materials
Issue number4
StatePublished - Apr 2021


  • Apigenin
  • Dental caries
  • Resin cement
  • Resin composite
  • Streptococcus mutans
  • tt-Farnesol

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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