Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)

Mark Zabriskie, Hengmiao Cheng, John C. Vederas

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Abstract

Incubation of D-phenylalanyl-L-phenylalanylglycine with peptidylglycine α-amidating monooxygenase (PAM) in the presence of 18O 2 generates the corresponding hydroxyglycyl peptide whose FAB mass spectrum shows high incorporation of 18O into the hydroxy group; this suggests that the mechanism may involve direct hydroxylation of carbon.

Original languageEnglish (US)
Pages (from-to)571-572
Number of pages2
JournalJournal of the Chemical Society, Chemical Communications
Issue number8
DOIs
Publication statusPublished - 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Medicine

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