Inactive X chromosome DNA does not function in DNA-mediated cell transformation for the hypoxanthine phosphoribosyltransferase gene.

R. M. Liskay, R. J. Evans

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The molecular nature of the X chromosome inactivation process has been investigated by utilizing the techniques of DNA-mediated cell transformation of the X-linked hypoxanthine phosphoribosyltransferase (HPRT) locus. The findings indicate that purified DNA from the inactive X chromosome of a near-euploid mouse cell line is not functional in transformation for HPRT, but the DNA from its "homologous" active X readily elicits transformation for HPRT in the same hamster cell recipient. These findings suggest that there is a difference between the DNA, per se, of the active and inactive X at (or near) the HPRT locus and that this difference could account, at least in part, for its inactivation.

Original languageEnglish (US)
Pages (from-to)4895-4898
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number8
DOIs
StatePublished - Aug 1980

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