Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice

Joni M. Mäki, Juha Rasanen, Hilkka Tikkanen, Raija Sormunen, Kaarin Mäkikallio, Kari I. Kivirikko, Raija Soininen

Research output: Contribution to journalArticle

322 Citations (Scopus)

Abstract

Background - The lysyl oxidases are extracellular copper enzymes that initiate the crosslinking of collagens and elastin. 5 human isoenzymes having been characterized so far. The crosslinks formed provide the tensile strength and elastic properties for various extracellular matrices, including vascular walls. We studied the role of the first described isoenzyme Lox by inactivating its gene in mice. Methods and Results - Murine Lox gene was disrupted by routine methods. Lox-/- mice died at the end of gestation or as neonates, necropsy of the live-born pups revealing large aortic aneurysms. In light microscopy, hazy and unruffled elastic lamellae in the Lox-/- aortas were observed, and electron microscopy of the aortic walls of the Lox-/- fetuses showed highly fragmented elastic fibers and discontinuity in the smooth muscle cell layers in Lox-/- fetuses. The wall of the aorta in the Lox-/- fetuses was significantly thicker, and the diameter of the aortic lumen was significantly smaller than that in the Lox+/+ aortas. In Lox-/- fetuses. Doppler ultrasonography revealed increased impedance in the umbilical artery, descending aorta, and intracranial artery blood velocity waveforms, decreased mean velocities across cardiac inflow and outflow regions, and increased pulsatility in ductus venosus blood velocity waveforms. Conclusions - Lox has an essential role in the development and function of the cardiovascular system. Inactivation of the Lox gene causes structural alterations in the arterial walls, leading to abnormalities in the cardiovascular functions. Alterations in LOX activity may also play a critical role in certain human cardiovascular diseases.

Original languageEnglish (US)
Pages (from-to)2503-2509
Number of pages7
JournalCirculation
Volume106
Issue number19
DOIs
StatePublished - Nov 5 2002
Externally publishedYes

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Protein-Lysine 6-Oxidase
Aortic Aneurysm
Fetus
Aorta
Isoenzymes
Genes
Cardiovascular Abnormalities
Doppler Ultrasonography
Umbilical Arteries
Elastic Tissue
Elastin
Tensile Strength
Gene Silencing
Cardiovascular System
Electric Impedance
Thoracic Aorta
Smooth Muscle Myocytes
Extracellular Matrix
Blood Vessels
Copper

Keywords

  • Aneurysm
  • Aorta
  • Crosslink
  • Lysyl oxidase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Mäki, J. M., Rasanen, J., Tikkanen, H., Sormunen, R., Mäkikallio, K., Kivirikko, K. I., & Soininen, R. (2002). Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice. Circulation, 106(19), 2503-2509. https://doi.org/10.1161/01.CIR.0000038109.84500.1E

Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice. / Mäki, Joni M.; Rasanen, Juha; Tikkanen, Hilkka; Sormunen, Raija; Mäkikallio, Kaarin; Kivirikko, Kari I.; Soininen, Raija.

In: Circulation, Vol. 106, No. 19, 05.11.2002, p. 2503-2509.

Research output: Contribution to journalArticle

Mäki, JM, Rasanen, J, Tikkanen, H, Sormunen, R, Mäkikallio, K, Kivirikko, KI & Soininen, R 2002, 'Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice', Circulation, vol. 106, no. 19, pp. 2503-2509. https://doi.org/10.1161/01.CIR.0000038109.84500.1E
Mäki, Joni M. ; Rasanen, Juha ; Tikkanen, Hilkka ; Sormunen, Raija ; Mäkikallio, Kaarin ; Kivirikko, Kari I. ; Soininen, Raija. / Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice. In: Circulation. 2002 ; Vol. 106, No. 19. pp. 2503-2509.
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abstract = "Background - The lysyl oxidases are extracellular copper enzymes that initiate the crosslinking of collagens and elastin. 5 human isoenzymes having been characterized so far. The crosslinks formed provide the tensile strength and elastic properties for various extracellular matrices, including vascular walls. We studied the role of the first described isoenzyme Lox by inactivating its gene in mice. Methods and Results - Murine Lox gene was disrupted by routine methods. Lox-/- mice died at the end of gestation or as neonates, necropsy of the live-born pups revealing large aortic aneurysms. In light microscopy, hazy and unruffled elastic lamellae in the Lox-/- aortas were observed, and electron microscopy of the aortic walls of the Lox-/- fetuses showed highly fragmented elastic fibers and discontinuity in the smooth muscle cell layers in Lox-/- fetuses. The wall of the aorta in the Lox-/- fetuses was significantly thicker, and the diameter of the aortic lumen was significantly smaller than that in the Lox+/+ aortas. In Lox-/- fetuses. Doppler ultrasonography revealed increased impedance in the umbilical artery, descending aorta, and intracranial artery blood velocity waveforms, decreased mean velocities across cardiac inflow and outflow regions, and increased pulsatility in ductus venosus blood velocity waveforms. Conclusions - Lox has an essential role in the development and function of the cardiovascular system. Inactivation of the Lox gene causes structural alterations in the arterial walls, leading to abnormalities in the cardiovascular functions. Alterations in LOX activity may also play a critical role in certain human cardiovascular diseases.",
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T1 - Inactivation of the lysyl oxidase gene Lox leads to aortic aneurysms, cardiovascular dysfunction, and perinatal death in mice

AU - Mäki, Joni M.

AU - Rasanen, Juha

AU - Tikkanen, Hilkka

AU - Sormunen, Raija

AU - Mäkikallio, Kaarin

AU - Kivirikko, Kari I.

AU - Soininen, Raija

PY - 2002/11/5

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N2 - Background - The lysyl oxidases are extracellular copper enzymes that initiate the crosslinking of collagens and elastin. 5 human isoenzymes having been characterized so far. The crosslinks formed provide the tensile strength and elastic properties for various extracellular matrices, including vascular walls. We studied the role of the first described isoenzyme Lox by inactivating its gene in mice. Methods and Results - Murine Lox gene was disrupted by routine methods. Lox-/- mice died at the end of gestation or as neonates, necropsy of the live-born pups revealing large aortic aneurysms. In light microscopy, hazy and unruffled elastic lamellae in the Lox-/- aortas were observed, and electron microscopy of the aortic walls of the Lox-/- fetuses showed highly fragmented elastic fibers and discontinuity in the smooth muscle cell layers in Lox-/- fetuses. The wall of the aorta in the Lox-/- fetuses was significantly thicker, and the diameter of the aortic lumen was significantly smaller than that in the Lox+/+ aortas. In Lox-/- fetuses. Doppler ultrasonography revealed increased impedance in the umbilical artery, descending aorta, and intracranial artery blood velocity waveforms, decreased mean velocities across cardiac inflow and outflow regions, and increased pulsatility in ductus venosus blood velocity waveforms. Conclusions - Lox has an essential role in the development and function of the cardiovascular system. Inactivation of the Lox gene causes structural alterations in the arterial walls, leading to abnormalities in the cardiovascular functions. Alterations in LOX activity may also play a critical role in certain human cardiovascular diseases.

AB - Background - The lysyl oxidases are extracellular copper enzymes that initiate the crosslinking of collagens and elastin. 5 human isoenzymes having been characterized so far. The crosslinks formed provide the tensile strength and elastic properties for various extracellular matrices, including vascular walls. We studied the role of the first described isoenzyme Lox by inactivating its gene in mice. Methods and Results - Murine Lox gene was disrupted by routine methods. Lox-/- mice died at the end of gestation or as neonates, necropsy of the live-born pups revealing large aortic aneurysms. In light microscopy, hazy and unruffled elastic lamellae in the Lox-/- aortas were observed, and electron microscopy of the aortic walls of the Lox-/- fetuses showed highly fragmented elastic fibers and discontinuity in the smooth muscle cell layers in Lox-/- fetuses. The wall of the aorta in the Lox-/- fetuses was significantly thicker, and the diameter of the aortic lumen was significantly smaller than that in the Lox+/+ aortas. In Lox-/- fetuses. Doppler ultrasonography revealed increased impedance in the umbilical artery, descending aorta, and intracranial artery blood velocity waveforms, decreased mean velocities across cardiac inflow and outflow regions, and increased pulsatility in ductus venosus blood velocity waveforms. Conclusions - Lox has an essential role in the development and function of the cardiovascular system. Inactivation of the Lox gene causes structural alterations in the arterial walls, leading to abnormalities in the cardiovascular functions. Alterations in LOX activity may also play a critical role in certain human cardiovascular diseases.

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