In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Per Haberkant, Reinout Raijmakers, Marjolein Wildwater, Timo Sachsenheimer, Britta Brügger, Kenji Maeda, Martin Houweling, Anne Claude Gavin, Carsten Schultz, Gerrit Van Meer, Albert J R Heck, Joost C M Holthuis

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Bifunctional lipid technology: Cells convert externally added photoactivatable and "clickable" fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound proteins in situ (see scheme).

Original languageEnglish (US)
Pages (from-to)4033-4038
Number of pages6
JournalAngewandte Chemie - International Edition
Volume52
Issue number14
DOIs
Publication statusPublished - Apr 2 2013
Externally publishedYes

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Keywords

  • cell imaging
  • click chemistry
  • lipids
  • photoaffinity labeling
  • proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Haberkant, P., Raijmakers, R., Wildwater, M., Sachsenheimer, T., Brügger, B., Maeda, K., ... Holthuis, J. C. M. (2013). In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. Angewandte Chemie - International Edition, 52(14), 4033-4038. https://doi.org/10.1002/anie.201210178