In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Per Haberkant, Reinout Raijmakers, Marjolein Wildwater, Timo Sachsenheimer, Britta Brügger, Kenji Maeda, Martin Houweling, Anne Claude Gavin, Carsten Schultz, Gerrit Van Meer, Albert J.R. Heck, Joost C.M. Holthuis

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Bifunctional lipid technology: Cells convert externally added photoactivatable and "clickable" fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound proteins in situ (see scheme).

Original languageEnglish (US)
Pages (from-to)4033-4038
Number of pages6
JournalAngewandte Chemie - International Edition
Volume52
Issue number14
DOIs
StatePublished - Apr 2 2013

Keywords

  • cell imaging
  • click chemistry
  • lipids
  • photoaffinity labeling
  • proteins

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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  • Cite this

    Haberkant, P., Raijmakers, R., Wildwater, M., Sachsenheimer, T., Brügger, B., Maeda, K., Houweling, M., Gavin, A. C., Schultz, C., Van Meer, G., Heck, A. J. R., & Holthuis, J. C. M. (2013). In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. Angewandte Chemie - International Edition, 52(14), 4033-4038. https://doi.org/10.1002/anie.201210178