TY - JOUR
T1 - In vivo Optical reflectance imaging of Spreading depression waves in rat brain with and without focal cerebral ischemia
AU - Chen, Shangbin
AU - Feng, Zhe
AU - Li, Pengcheng
AU - Jacques, Steven L.
AU - Zeng, Shaoqun
AU - Luo, Qingming
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (grant number 60478016), Major Program of Science and Technology Research of Ministry of Education (grant number 10420), Joint Research Fund for Overseas Chinese Young Scholars (grant number 30328014), and the National Institutes of Health (R01-EB000224). The help of Ralf B. Schulz in revising the manuscript is gratefully acknowledged.
PY - 2006/5
Y1 - 2006/5
N2 - Spreading depression (SD) waves occur in focal cerebral ischemia of the brain. Optical reflectance imaging at 550±10-nm wavelength using a charge-coupled device (CCD) camera, called optical intrinsic signal imaging (OISI) in the neuroscience community, provides high resolution imaging of SD waves based on changes in blood perfusion. We present optical images of SD waves in normal rat brain induced by a pinprick, and the spontaneous SD waves that follow middle cerebral artery occlusion (MCAO). The images of change in reflectance are calculated as A=(I-Io)/Io, where I is pixel intensity as some timepoint and Io is the initial intensity just prior to an SD wave. Difference images B = [I(i)-I(i-1)]/Io, where I(i) is the image at time i and I(i-1) is the previous image at time i-1 (a 6.4-s interval), significantly sharpen the boundaries between leading and trailing edges of the SD wave. Maximum rate-of-change images C=max(B) display the maximum pixel value of B within the duration of a single SD wave, and provide an image that visualizes the entire penumbra. The penumbra appear bright due to a rapid drop in perfusion, while the normal brain and infarct area appear dark.
AB - Spreading depression (SD) waves occur in focal cerebral ischemia of the brain. Optical reflectance imaging at 550±10-nm wavelength using a charge-coupled device (CCD) camera, called optical intrinsic signal imaging (OISI) in the neuroscience community, provides high resolution imaging of SD waves based on changes in blood perfusion. We present optical images of SD waves in normal rat brain induced by a pinprick, and the spontaneous SD waves that follow middle cerebral artery occlusion (MCAO). The images of change in reflectance are calculated as A=(I-Io)/Io, where I is pixel intensity as some timepoint and Io is the initial intensity just prior to an SD wave. Difference images B = [I(i)-I(i-1)]/Io, where I(i) is the image at time i and I(i-1) is the previous image at time i-1 (a 6.4-s interval), significantly sharpen the boundaries between leading and trailing edges of the SD wave. Maximum rate-of-change images C=max(B) display the maximum pixel value of B within the duration of a single SD wave, and provide an image that visualizes the entire penumbra. The penumbra appear bright due to a rapid drop in perfusion, while the normal brain and infarct area appear dark.
KW - Focal cerebral ischemia
KW - Middle cerebral artery occlusion
KW - Optical intrinsic signal imaging
KW - Spreading depression
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U2 - 10.1117/1.2203654
DO - 10.1117/1.2203654
M3 - Article
C2 - 16822052
AN - SCOPUS:33748448115
SN - 1083-3668
VL - 11
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 3
M1 - 034002
ER -