In vivo optical imaging of revascularization after brain trauma in mice

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Revascularization following brain trauma is crucial to the repair process. We used optical micro-angiography (OMAG) to study endogenous revascularization in living mice following brain injury. OMAG is a volumetric optical imaging method capable of in vivo mapping of localized blood perfusion within the scanned tissue beds down to capillary level imaging resolution. We demonstrated that OMAG can differentiate revascularization progression between traumatized mice with and without soluble epoxide hydrolase (sEH) gene deletion. The time course of revascularization was determined from serial imaging of the traumatic region in the same mice over a one-month period of rehabilitation. Restoration of blood volume at the lesion site was more pronounced in sEH knockout mice than in wild-type mice as determined by OMAG. These OMAG measurements were confirmed by histology and showed that the sEH knockout effect may be involved in enhancing revascularization. The correlation of OMAG with histology also suggests that OMAG is a useful imaging tool for real-time in vivo monitoring of post-traumatic revascularization and for evaluating agents that inhibit or promote endogenous revascularization during the recovery process in small rodents.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalMicrovascular Research
Volume81
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Angiography
Optical Imaging
Brain
Imaging techniques
Epoxide Hydrolases
Histology
Blood
Gene Deletion
Traumatic Brain Injury
Blood Volume
Knockout Mice
Patient rehabilitation
Brain Injuries
Restoration
Rodentia
Repair
Rehabilitation
Perfusion
Genes
Tissue

Keywords

  • Cerebral blood flow
  • EETs
  • Optical imaging
  • Revascularization
  • Soluble epoxide hydrolase
  • Traumatic brain injury

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

In vivo optical imaging of revascularization after brain trauma in mice. / Yali, Jia; Grafe, Marjorie; Gruber, Andras; Alkayed, Nabil; Wang, Ruikang K.

In: Microvascular Research, Vol. 81, No. 1, 01.2011, p. 73-80.

Research output: Contribution to journalArticle

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