TY - JOUR
T1 - In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction
AU - Ly, Hung Q.
AU - Hoshino, Kozo
AU - Pomerantseva, Irina
AU - Kawase, Yoshiaki
AU - Yoneyama, Ryuichi
AU - Takewa, Yoshiaki
AU - Fortier, Annik
AU - Gibbs-Strauss, Summer L.
AU - Vooght, Carrie
AU - Frangioni, John V.
AU - Hajjar, Roger J.
N1 - Funding Information:
This study was supported by grants from the National Institutes of Health: R01-CA-115296 (J.V.F.) and R01 HL078691, HL057263, HL071763, HL080498, and HL083156, and a Leducq Transatlantic Network (R.J.H.).
PY - 2009/12
Y1 - 2009/12
N2 - Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.
AB - Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.
KW - In vivo
KW - Intracoronary
KW - Multipotent progenitor cells
KW - Myocardial infarction
KW - Near-infrared fluorescence
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U2 - 10.1093/eurheartj/ehp322
DO - 10.1093/eurheartj/ehp322
M3 - Article
C2 - 19687154
AN - SCOPUS:71549165176
SN - 0195-668X
VL - 30
SP - 2861
EP - 2868
JO - European heart journal
JF - European heart journal
IS - 23
ER -