In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction

Hung Q. Ly, Kozo Hoshino, Irina Pomerantseva, Yoshiaki Kawase, Ryuichi Yoneyama, Yoshiaki Takewa, Annik Fortier, Summer Gibbs, Carrie Vooght, John V. Frangioni, Roger J. Hajjar

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.

Original languageEnglish (US)
Pages (from-to)2861-2868
Number of pages8
JournalEuropean Heart Journal
Volume30
Issue number23
DOIs
StatePublished - Dec 2009
Externally publishedYes

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Swine
Stem Cells
Myocardial Infarction
Fluorescence
Cell- and Tissue-Based Therapy
Injections
Bone Marrow
Mesenchymal Stromal Cells
Blood Cells
Population

Keywords

  • In vivo
  • Intracoronary
  • Multipotent progenitor cells
  • Myocardial infarction
  • Near-infrared fluorescence

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction. / Ly, Hung Q.; Hoshino, Kozo; Pomerantseva, Irina; Kawase, Yoshiaki; Yoneyama, Ryuichi; Takewa, Yoshiaki; Fortier, Annik; Gibbs, Summer; Vooght, Carrie; Frangioni, John V.; Hajjar, Roger J.

In: European Heart Journal, Vol. 30, No. 23, 12.2009, p. 2861-2868.

Research output: Contribution to journalArticle

Ly, HQ, Hoshino, K, Pomerantseva, I, Kawase, Y, Yoneyama, R, Takewa, Y, Fortier, A, Gibbs, S, Vooght, C, Frangioni, JV & Hajjar, RJ 2009, 'In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction', European Heart Journal, vol. 30, no. 23, pp. 2861-2868. https://doi.org/10.1093/eurheartj/ehp322
Ly, Hung Q. ; Hoshino, Kozo ; Pomerantseva, Irina ; Kawase, Yoshiaki ; Yoneyama, Ryuichi ; Takewa, Yoshiaki ; Fortier, Annik ; Gibbs, Summer ; Vooght, Carrie ; Frangioni, John V. ; Hajjar, Roger J. / In vivo myocardial distribution of multipotent progenitor cells following intracoronary delivery in a swine model of myocardial infarction. In: European Heart Journal. 2009 ; Vol. 30, No. 23. pp. 2861-2868.
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abstract = "Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.",
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AU - Ly, Hung Q.

AU - Hoshino, Kozo

AU - Pomerantseva, Irina

AU - Kawase, Yoshiaki

AU - Yoneyama, Ryuichi

AU - Takewa, Yoshiaki

AU - Fortier, Annik

AU - Gibbs, Summer

AU - Vooght, Carrie

AU - Frangioni, John V.

AU - Hajjar, Roger J.

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N2 - Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.

AB - Aims There are few data comparing the fate of multipotent progenitor cells (MPCs) used in cardiac cell therapy after myocardial infarction (MI). To document in vivo distribution of MPCs delivered by intracoronary (IC) injection.Methods and resultsUsing an anterior MI swine model, near-infrared (NIR) fluorescence was used for in vivo tracking of labelled MPCs [mesenchymal stromal (MSCs), bone marrow mononuclear (BMMNCs), and peripheral blood mononuclear (PBMNCs)] cells early after IC injection. Signal intensity ratios (SIRs) of injected over non-injected (reference) zones were used to report NIR fluorescence emission. Following IC injection, significant differences in mean SIR were documented when MSCs were compared with BMMNCs [1.28 ± 0.10 vs. 0.77 ± 0.11, P < 0.001; 95 CI (0.219, 0.805), respectively] or PBMNCs [1.28 ± 0.10 vs. 0.80 ± 0.14, P = 0.005; 95 CI (0.148, 0.813), respectively]. Differences were maintained during the 60 min tracking period, with only the MSC-injected groups continuously emitting NIR fluorescence (SIR>1). This is correlated with greater cell retention for MSCs relative to mononuclear cells. However, there was evidence of MSC-related vessel plugging in some swine.ConclusionOur in vivo NIR fluorescence findings suggest that MPC distribution and retention immediately after intracoronary delivery vary depending on cell population and could potentially impact the clinical efficacy of cardiac cell therapy.

KW - In vivo

KW - Intracoronary

KW - Multipotent progenitor cells

KW - Myocardial infarction

KW - Near-infrared fluorescence

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