In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats: II. Inhibition by γ-aminobutyric acid

Pedro V. Rada, Gregory P. Mark, Bartley G. Hoebel

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Our earlier studies suggest dopamine and serotonin interact with acetylcholine (ACh) in the nucleus accumbens (NAC) as part of a system for motivation and reinforcement. The purpose of the present experiment was to characterize a possible link between GABA and acetylcholine in the nucleus accumbens using microdialysis in freely moving rats. Different doses of GABA, muscimol, baclofen, saclofen and bicuculline were locally infused into the nucleus accumbens through the microdialysis probe. GABA and its agonists dose-dependently decreased extracellular levels of acetylcholine in the nucleus accumbens. In contrast the GABAA antagonist, bicuculinne, dose-dependently increased extracellular ACh while the GABAB antagonist, saclofen, was without effect. Co-infusion of bicuculline or saclofen was shown to block the decrease in recoverable ACh produced by muscimol or baclofen, respectively. The results demonstrate an inhibitory action of GABA on acetylcholine interneurones in the nucleus accumbens involving both GABAA and GABAB receptor subtypes. In addition a tonic inhibitory GABAergic tone is probably mediated through GABAA receptors.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalBrain research
Volume619
Issue number1-2
DOIs
StatePublished - Aug 13 1993

Keywords

  • Acetylcholine
  • Baclofen
  • Bicuculline
  • GABA
  • Microdialysis
  • Muscimol
  • Nucleus accumbens
  • Saclofen

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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