In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats: I. Inhibition by serotonin

Pedro V. Rada, Gregory Mark, Bartley G. Hoebel

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Microdialysis was used to characterize the effect of serotonergic input on cholinergic interneurons in the nucleus accumbens (NAC) of freely moving rats. Local infusion of 5-hydroxytryptamine (5-HT) or the serotonin reuptake blocker fluoxetine significantly decreased extracellular acetylcholine (ACh) in the NAC. This decrease in ACh was blocked by the 5-HT1 (and β-adrenergic) antagonist propranolol. To test β-adrenergic effects, it was found that a β-adrenergic agonist isoproterenol had no measurable effect on extracellular ACh in the NAC. This suggests that 5-HT inhibits ACh interneurons via one of the 5-HT1 receptor types. The 5-HT1A agonist 8-OH-DPAT given systematically again decreased extracellular levels of ACh, and the effect was dose-dependent. The 5-HT1A effect was probably exerted in the NAC, because local infusion of 8-OH-DPAT mimicked systemic injections. These microdialysis results are similar to in vitro studies which suggest an inhibitory impact of 5-HT on ACh release in basal ganglia slices and homogenates. The decrease in extracellular ACh as measured in vivo is apparently mediated, at least in part, through a 5-HT1A receptor in the accumbens. Given the role of the NAC in behavior reinforcement, this 5HT-ACh interaction may be involved in serotonergic treatment of depression.

Original languageEnglish (US)
Pages (from-to)98-104
Number of pages7
JournalBrain Research
Volume619
Issue number1-2
DOIs
StatePublished - Aug 13 1993
Externally publishedYes

Fingerprint

Nucleus Accumbens
Acetylcholine
Serotonin
8-Hydroxy-2-(di-n-propylamino)tetralin
Microdialysis
Interneurons
Serotonin 5-HT1 Receptors
Serotonin 5-HT1 Receptor Antagonists
Serotonin 5-HT1 Receptor Agonists
Adrenergic Agonists
Receptor, Serotonin, 5-HT1A
Adrenergic Antagonists
Inhibition (Psychology)
Fluoxetine
Basal Ganglia
Isoproterenol
Propranolol
Adrenergic Agents
Cholinergic Agents
Depression

Keywords

  • 8-OH-DPAT
  • Acetylcholine
  • Fluoxetine
  • Microdialysis
  • Nucleus accumbens
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats : I. Inhibition by serotonin. / Rada, Pedro V.; Mark, Gregory; Hoebel, Bartley G.

In: Brain Research, Vol. 619, No. 1-2, 13.08.1993, p. 98-104.

Research output: Contribution to journalArticle

Rada, Pedro V. ; Mark, Gregory ; Hoebel, Bartley G. / In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats : I. Inhibition by serotonin. In: Brain Research. 1993 ; Vol. 619, No. 1-2. pp. 98-104.
@article{5afc71e197e34dc1b2a12abcba96e3b2,
title = "In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats: I. Inhibition by serotonin",
abstract = "Microdialysis was used to characterize the effect of serotonergic input on cholinergic interneurons in the nucleus accumbens (NAC) of freely moving rats. Local infusion of 5-hydroxytryptamine (5-HT) or the serotonin reuptake blocker fluoxetine significantly decreased extracellular acetylcholine (ACh) in the NAC. This decrease in ACh was blocked by the 5-HT1 (and β-adrenergic) antagonist propranolol. To test β-adrenergic effects, it was found that a β-adrenergic agonist isoproterenol had no measurable effect on extracellular ACh in the NAC. This suggests that 5-HT inhibits ACh interneurons via one of the 5-HT1 receptor types. The 5-HT1A agonist 8-OH-DPAT given systematically again decreased extracellular levels of ACh, and the effect was dose-dependent. The 5-HT1A effect was probably exerted in the NAC, because local infusion of 8-OH-DPAT mimicked systemic injections. These microdialysis results are similar to in vitro studies which suggest an inhibitory impact of 5-HT on ACh release in basal ganglia slices and homogenates. The decrease in extracellular ACh as measured in vivo is apparently mediated, at least in part, through a 5-HT1A receptor in the accumbens. Given the role of the NAC in behavior reinforcement, this 5HT-ACh interaction may be involved in serotonergic treatment of depression.",
keywords = "8-OH-DPAT, Acetylcholine, Fluoxetine, Microdialysis, Nucleus accumbens, Serotonin",
author = "Rada, {Pedro V.} and Gregory Mark and Hoebel, {Bartley G.}",
year = "1993",
month = "8",
day = "13",
doi = "10.1016/0006-8993(93)91600-W",
language = "English (US)",
volume = "619",
pages = "98--104",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - In vivo modulation of acetylcholine in the nucleus accumbens of freely moving rats

T2 - I. Inhibition by serotonin

AU - Rada, Pedro V.

AU - Mark, Gregory

AU - Hoebel, Bartley G.

PY - 1993/8/13

Y1 - 1993/8/13

N2 - Microdialysis was used to characterize the effect of serotonergic input on cholinergic interneurons in the nucleus accumbens (NAC) of freely moving rats. Local infusion of 5-hydroxytryptamine (5-HT) or the serotonin reuptake blocker fluoxetine significantly decreased extracellular acetylcholine (ACh) in the NAC. This decrease in ACh was blocked by the 5-HT1 (and β-adrenergic) antagonist propranolol. To test β-adrenergic effects, it was found that a β-adrenergic agonist isoproterenol had no measurable effect on extracellular ACh in the NAC. This suggests that 5-HT inhibits ACh interneurons via one of the 5-HT1 receptor types. The 5-HT1A agonist 8-OH-DPAT given systematically again decreased extracellular levels of ACh, and the effect was dose-dependent. The 5-HT1A effect was probably exerted in the NAC, because local infusion of 8-OH-DPAT mimicked systemic injections. These microdialysis results are similar to in vitro studies which suggest an inhibitory impact of 5-HT on ACh release in basal ganglia slices and homogenates. The decrease in extracellular ACh as measured in vivo is apparently mediated, at least in part, through a 5-HT1A receptor in the accumbens. Given the role of the NAC in behavior reinforcement, this 5HT-ACh interaction may be involved in serotonergic treatment of depression.

AB - Microdialysis was used to characterize the effect of serotonergic input on cholinergic interneurons in the nucleus accumbens (NAC) of freely moving rats. Local infusion of 5-hydroxytryptamine (5-HT) or the serotonin reuptake blocker fluoxetine significantly decreased extracellular acetylcholine (ACh) in the NAC. This decrease in ACh was blocked by the 5-HT1 (and β-adrenergic) antagonist propranolol. To test β-adrenergic effects, it was found that a β-adrenergic agonist isoproterenol had no measurable effect on extracellular ACh in the NAC. This suggests that 5-HT inhibits ACh interneurons via one of the 5-HT1 receptor types. The 5-HT1A agonist 8-OH-DPAT given systematically again decreased extracellular levels of ACh, and the effect was dose-dependent. The 5-HT1A effect was probably exerted in the NAC, because local infusion of 8-OH-DPAT mimicked systemic injections. These microdialysis results are similar to in vitro studies which suggest an inhibitory impact of 5-HT on ACh release in basal ganglia slices and homogenates. The decrease in extracellular ACh as measured in vivo is apparently mediated, at least in part, through a 5-HT1A receptor in the accumbens. Given the role of the NAC in behavior reinforcement, this 5HT-ACh interaction may be involved in serotonergic treatment of depression.

KW - 8-OH-DPAT

KW - Acetylcholine

KW - Fluoxetine

KW - Microdialysis

KW - Nucleus accumbens

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=0027337033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027337033&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(93)91600-W

DO - 10.1016/0006-8993(93)91600-W

M3 - Article

C2 - 8374797

AN - SCOPUS:0027337033

VL - 619

SP - 98

EP - 104

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -