In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents

Kevin G. Phillips, Ravikant Samatham, Philippe Thuillier, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using a reflectance mode confocal scanning laser microscope (rCSLM), a noninvasive technique to monitor epidermal thickness in vivo is presented. The modality is characterized by the collection of the reflectance profile from cutaneous tissues, resolved in transverse (x, y) directions at each depth (z) increment. Due to the different light scattering properties of the skin layers, each layer can be identified. The depth of each layer can then be inferred from the axial reflectance profile along the z direction. In pilot experiments an agent that induced epidermal proliferation, 12-O-tetradecanoylphorbol-13-acetate (TPA), was applied topically to the ear of a mouse. Peak-to-valley analysis of the rCSLM A-scans showed the epidermal thickness increasing from an initial 5.4 [μm] to 12.4 [μm] after 24 [hr]. Peak-to-peak analysis showed an increase from 9.1 [μm] to 21.2 [μm]. These results suggest that rCSLM imaging provides a means to study morphologic changes in the epithelium arising from inflamatory response and cell proliferation in vivo without recourse to biopsy or sacrifice of animals.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7187
DOIs
StatePublished - 2009
EventBiomedical Applications of Light Scattering III - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Other

OtherBiomedical Applications of Light Scattering III
CountryUnited States
CitySan Jose, CA
Period1/24/091/26/09

Fingerprint

Confocal microscopy
Laser modes
Confocal Microscopy
Lasers
Cell Proliferation
microscopy
reflectance
Microscopes
microscopes
Skin
Scanning
scanning
Tetradecanoylphorbol Acetate
Ear
lasers
Epithelium
epithelium
Biopsy
Cell proliferation
Light

Keywords

  • Cancer
  • Confocal microscopy
  • Epidermal thickness
  • Inflammatory response
  • Optical coherence tomography

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Phillips, K. G., Samatham, R., Thuillier, P., & Jacques, S. (2009). In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7187). [71870S] https://doi.org/10.1117/12.809674

In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents. / Phillips, Kevin G.; Samatham, Ravikant; Thuillier, Philippe; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7187 2009. 71870S.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Phillips, KG, Samatham, R, Thuillier, P & Jacques, S 2009, In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7187, 71870S, Biomedical Applications of Light Scattering III, San Jose, CA, United States, 1/24/09. https://doi.org/10.1117/12.809674
Phillips KG, Samatham R, Thuillier P, Jacques S. In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7187. 2009. 71870S https://doi.org/10.1117/12.809674
Phillips, Kevin G. ; Samatham, Ravikant ; Thuillier, Philippe ; Jacques, Steven. / In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7187 2009.
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