In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models

Kevin G. Phillips, Ravikant Samatham, Niloy Choudhury, James C. Gladish, Philippe Thuillier, Steven Jacques

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The characterization of tissue morphology in murine models of pathogenesis has traditionally been carried out by excision of affected tissues with subsequent immunohistological examination. Excision-based histology provides a limited two-dimensional presentation of tissue morphology at the cost of halting disease progression at a single time point and sacrifice of the animal. We investigate the use of noninvasive reflectance mode confocal scanning laser microscopy (rCSLM) as an alternative tool to biopsy in documenting epidermal hyperplasia in murine models exposed to the tumor promoter 12-O- tetradecanoylphorbol-13-acetate (TPA). An automated technique utilizing average axial rCSLM reflectance profiles is used to extract epidermal thickness values from rCSLM data cubes. In comparisons to epidermal thicknesses determined from hematoxylin and eosin (H&E) stained tissue sections, we find no significant correlation to rCSLM-derived thickness values. This results from method-specific artifacts: physical alterations of tissue during H&E preparation in standard histology and specimen-induced abberations in rCSLM imaging. Despite their disagreement, both histology and rCSLM methods reliably measure statistically significant thickness changes in response to TPA exposure. Our results demonstrate that in vivo rCSLM imaging provides epithelial biologists an accurate noninvasive means to monitor cutaneous pathogenesis

Original languageEnglish (US)
Article number041514
JournalJournal of Biomedical Optics
Volume15
Issue number4
DOIs
StatePublished - Jul 2010

Fingerprint

laser microscopy
promotion
Laser modes
Tumors
tumors
Microscopic examination
reflectance
Scanning
scanning
Lasers
Histology
histology
Tissue
pathogenesis
acetates
Acetates
Imaging techniques
Biopsy
Hematoxylin
Eosine Yellowish-(YS)

Keywords

  • Cancer
  • Confocal microscopy
  • Hyperplasia
  • Image segmentation
  • Noninvasive imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models. / Phillips, Kevin G.; Samatham, Ravikant; Choudhury, Niloy; Gladish, James C.; Thuillier, Philippe; Jacques, Steven.

In: Journal of Biomedical Optics, Vol. 15, No. 4, 041514, 07.2010.

Research output: Contribution to journalArticle

Phillips, Kevin G. ; Samatham, Ravikant ; Choudhury, Niloy ; Gladish, James C. ; Thuillier, Philippe ; Jacques, Steven. / In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 4.
@article{7ba7f18a95d74740a61e7a1afc76cd10,
title = "In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models",
abstract = "The characterization of tissue morphology in murine models of pathogenesis has traditionally been carried out by excision of affected tissues with subsequent immunohistological examination. Excision-based histology provides a limited two-dimensional presentation of tissue morphology at the cost of halting disease progression at a single time point and sacrifice of the animal. We investigate the use of noninvasive reflectance mode confocal scanning laser microscopy (rCSLM) as an alternative tool to biopsy in documenting epidermal hyperplasia in murine models exposed to the tumor promoter 12-O- tetradecanoylphorbol-13-acetate (TPA). An automated technique utilizing average axial rCSLM reflectance profiles is used to extract epidermal thickness values from rCSLM data cubes. In comparisons to epidermal thicknesses determined from hematoxylin and eosin (H&E) stained tissue sections, we find no significant correlation to rCSLM-derived thickness values. This results from method-specific artifacts: physical alterations of tissue during H&E preparation in standard histology and specimen-induced abberations in rCSLM imaging. Despite their disagreement, both histology and rCSLM methods reliably measure statistically significant thickness changes in response to TPA exposure. Our results demonstrate that in vivo rCSLM imaging provides epithelial biologists an accurate noninvasive means to monitor cutaneous pathogenesis",
keywords = "Cancer, Confocal microscopy, Hyperplasia, Image segmentation, Noninvasive imaging",
author = "Phillips, {Kevin G.} and Ravikant Samatham and Niloy Choudhury and Gladish, {James C.} and Philippe Thuillier and Steven Jacques",
year = "2010",
month = "7",
doi = "10.1117/1.3455508",
language = "English (US)",
volume = "15",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

TY - JOUR

T1 - In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models

AU - Phillips, Kevin G.

AU - Samatham, Ravikant

AU - Choudhury, Niloy

AU - Gladish, James C.

AU - Thuillier, Philippe

AU - Jacques, Steven

PY - 2010/7

Y1 - 2010/7

N2 - The characterization of tissue morphology in murine models of pathogenesis has traditionally been carried out by excision of affected tissues with subsequent immunohistological examination. Excision-based histology provides a limited two-dimensional presentation of tissue morphology at the cost of halting disease progression at a single time point and sacrifice of the animal. We investigate the use of noninvasive reflectance mode confocal scanning laser microscopy (rCSLM) as an alternative tool to biopsy in documenting epidermal hyperplasia in murine models exposed to the tumor promoter 12-O- tetradecanoylphorbol-13-acetate (TPA). An automated technique utilizing average axial rCSLM reflectance profiles is used to extract epidermal thickness values from rCSLM data cubes. In comparisons to epidermal thicknesses determined from hematoxylin and eosin (H&E) stained tissue sections, we find no significant correlation to rCSLM-derived thickness values. This results from method-specific artifacts: physical alterations of tissue during H&E preparation in standard histology and specimen-induced abberations in rCSLM imaging. Despite their disagreement, both histology and rCSLM methods reliably measure statistically significant thickness changes in response to TPA exposure. Our results demonstrate that in vivo rCSLM imaging provides epithelial biologists an accurate noninvasive means to monitor cutaneous pathogenesis

AB - The characterization of tissue morphology in murine models of pathogenesis has traditionally been carried out by excision of affected tissues with subsequent immunohistological examination. Excision-based histology provides a limited two-dimensional presentation of tissue morphology at the cost of halting disease progression at a single time point and sacrifice of the animal. We investigate the use of noninvasive reflectance mode confocal scanning laser microscopy (rCSLM) as an alternative tool to biopsy in documenting epidermal hyperplasia in murine models exposed to the tumor promoter 12-O- tetradecanoylphorbol-13-acetate (TPA). An automated technique utilizing average axial rCSLM reflectance profiles is used to extract epidermal thickness values from rCSLM data cubes. In comparisons to epidermal thicknesses determined from hematoxylin and eosin (H&E) stained tissue sections, we find no significant correlation to rCSLM-derived thickness values. This results from method-specific artifacts: physical alterations of tissue during H&E preparation in standard histology and specimen-induced abberations in rCSLM imaging. Despite their disagreement, both histology and rCSLM methods reliably measure statistically significant thickness changes in response to TPA exposure. Our results demonstrate that in vivo rCSLM imaging provides epithelial biologists an accurate noninvasive means to monitor cutaneous pathogenesis

KW - Cancer

KW - Confocal microscopy

KW - Hyperplasia

KW - Image segmentation

KW - Noninvasive imaging

UR - http://www.scopus.com/inward/record.url?scp=77954568780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954568780&partnerID=8YFLogxK

U2 - 10.1117/1.3455508

DO - 10.1117/1.3455508

M3 - Article

C2 - 20799792

AN - SCOPUS:77954568780

VL - 15

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 041514

ER -