In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity

influence of drug dose and tissue type

V. Vaidyanathan, Sohi Rastegar, T. W. Fossum, P. Flores, E. W. Breggen, N. G. Egger, Steven Jacques, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Fluorescence spectroscopic detection and photodynamic therapy may provide an effective approach for early detection and treatment of oral cancer. Thus the development of a safe photosensitizer that could enhance the spectroscopic contrast between normal and neoplastic tissue, while allowing for selective photosensitization and treatment of pre-malignant and malignant lesions in the oral cavity, is highly desired. In this study, the pharmacokinetics and a safety of 5-aminolevulinic acid (ALA) that could induce an endogenous precursor of protoporphyrin IX and heme in the biosynthetic pathway was investigated. Two doses of ALA:25 and 75 mg/kg were administered intravenously to 4 and 3 dogs, respectively. A 'wash-out' period of 1 week between administration of each does was allowed to ensure against PpIX build-up. Using an optical multichannel analyzer, the fluorescence from the oral cavity was recorded at 3 sites: buccal mucosa, gums, and the tongue, and also from a remote site, the skin. A fiber optic probe was used to deliver excitation and collect the emitted fluorescence. Results showed that the ALA-induced fluorescence reached a peak at 2-4 hours, and returned to baseline in 24-31 hours. The dogs were stable during the course of the study, minimal vomiting was noted. In conclusion, the study showed that higher doses result in a higher peak at a later time.It was observed that different tissues have different pharmacokinetic response, the tongue and the gums have the highest peak fluorescence values, followed by the buccal mucosa and skin.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages222-226
Number of pages5
Volume2975
ISBN (Print)0819423866
StatePublished - 1997
Externally publishedYes
EventLaser-Tissue Interaction VIII - San Jose, CA, USA
Duration: Feb 9 1997Feb 12 1997

Other

OtherLaser-Tissue Interaction VIII
CitySan Jose, CA, USA
Period2/9/972/12/97

Fingerprint

Dosimetry
drugs
Fluorescence
Tissue
dosage
fluorescence
cavities
acids
Kinetics
Acids
kinetics
Pharmacokinetics
tongue
dogs
Skin
vomiting
Photodynamic therapy
Photosensitizers
lesions
Fiber optics

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Vaidyanathan, V., Rastegar, S., Fossum, T. W., Flores, P., Breggen, E. W., Egger, N. G., ... Motamedi, M. (1997). In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity: influence of drug dose and tissue type. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2975, pp. 222-226). Society of Photo-Optical Instrumentation Engineers.

In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity : influence of drug dose and tissue type. / Vaidyanathan, V.; Rastegar, Sohi; Fossum, T. W.; Flores, P.; Breggen, E. W.; Egger, N. G.; Jacques, Steven; Motamedi, Massoud.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2975 Society of Photo-Optical Instrumentation Engineers, 1997. p. 222-226.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Vaidyanathan, V, Rastegar, S, Fossum, TW, Flores, P, Breggen, EW, Egger, NG, Jacques, S & Motamedi, M 1997, In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity: influence of drug dose and tissue type. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2975, Society of Photo-Optical Instrumentation Engineers, pp. 222-226, Laser-Tissue Interaction VIII, San Jose, CA, USA, 2/9/97.
Vaidyanathan V, Rastegar S, Fossum TW, Flores P, Breggen EW, Egger NG et al. In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity: influence of drug dose and tissue type. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2975. Society of Photo-Optical Instrumentation Engineers. 1997. p. 222-226
Vaidyanathan, V. ; Rastegar, Sohi ; Fossum, T. W. ; Flores, P. ; Breggen, E. W. ; Egger, N. G. ; Jacques, Steven ; Motamedi, Massoud. / In-vivo kinetics of ALA-induced fluorescence in the canine oral cavity : influence of drug dose and tissue type. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2975 Society of Photo-Optical Instrumentation Engineers, 1997. pp. 222-226
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