In vivo imaging of mouse tumors by a lipidated cathepsin S substrate

Hai Yu Hu, Divya Vats, Matej Vizovisek, Lovro Kramer, Catherine Germanier, K. Ulrich Wendt, Markus Rudin, Boris Turk, Oliver Plettenburg, Carsten Schultz

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The synthesis and evaluation of two cathepsin S-specific probes is described. For long-term retention of the probe at the target site and a high signal-to-noise ratio, we introduced a lipidation approach via the simple attachment of palmitoic acid to the reporter. After cathepsin S-specific cleavage in cultured cells and in a grafted tumor mouse model, fluorescence increased owing to dequenching and we observed an intracellular accumulation of the fluorescence in the target tissue. The lipidated probe provided a prolonged and strongly fluorescent signal in tumors when compared to the very similar non-lipidated probe, demonstrating that non-invasive tumor identification is feasable. The homing principle by probe lipidation might also work for selective administration of cytotoxic compounds to specifically reduce tumor mass.

Original languageEnglish (US)
Pages (from-to)7669-7673
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number29
DOIs
StatePublished - Jul 14 2014
Externally publishedYes

Fingerprint

cathepsin S
Tumors
Imaging techniques
Substrates
Fluorescence
Signal to noise ratio
Cells
Cathepsins
Tissue
Acids

Keywords

  • fluorescence probes
  • FRET
  • homing
  • lipidation
  • tumor diagnosis

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

In vivo imaging of mouse tumors by a lipidated cathepsin S substrate. / Hu, Hai Yu; Vats, Divya; Vizovisek, Matej; Kramer, Lovro; Germanier, Catherine; Wendt, K. Ulrich; Rudin, Markus; Turk, Boris; Plettenburg, Oliver; Schultz, Carsten.

In: Angewandte Chemie - International Edition, Vol. 53, No. 29, 14.07.2014, p. 7669-7673.

Research output: Contribution to journalArticle

Hu, HY, Vats, D, Vizovisek, M, Kramer, L, Germanier, C, Wendt, KU, Rudin, M, Turk, B, Plettenburg, O & Schultz, C 2014, 'In vivo imaging of mouse tumors by a lipidated cathepsin S substrate', Angewandte Chemie - International Edition, vol. 53, no. 29, pp. 7669-7673. https://doi.org/10.1002/anie.201310979
Hu HY, Vats D, Vizovisek M, Kramer L, Germanier C, Wendt KU et al. In vivo imaging of mouse tumors by a lipidated cathepsin S substrate. Angewandte Chemie - International Edition. 2014 Jul 14;53(29):7669-7673. https://doi.org/10.1002/anie.201310979
Hu, Hai Yu ; Vats, Divya ; Vizovisek, Matej ; Kramer, Lovro ; Germanier, Catherine ; Wendt, K. Ulrich ; Rudin, Markus ; Turk, Boris ; Plettenburg, Oliver ; Schultz, Carsten. / In vivo imaging of mouse tumors by a lipidated cathepsin S substrate. In: Angewandte Chemie - International Edition. 2014 ; Vol. 53, No. 29. pp. 7669-7673.
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