In vivo imaging of coral tissue and skeleton with optical coherence tomography

Daniel Wangpraseurt, Camilla Wentzel, Steven Jacques, Michael Wagner, Michael Kühl

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Application of optical coherence tomography (OCT) for in vivo imaging of tissue and skeleton structure of intact living corals enabled the non-invasive visualization of coral tissue layers (endoderm versus ectoderm), skeletal cavities and special structures such as mesenterial filaments and mucus release from intact living corals. Coral host chromatophores containing green fluorescent protein-like pigment granules appeared hyper-reflective to near-infrared radiation allowing for excellent optical contrast in OCT and a rapid characterization of chromatophore size, distribution and abundance. In vivo tissue plasticity could be quantified by the linear contraction velocity of coral tissues upon illumination resulting in dynamic changes in the live coral tissue surface area, which varied by a factor of 2 between the contracted and expanded state of a coral. Our study provides a novel view on the in vivo organization of coral tissue and skeleton and highlights the importance of microstructural dynamics for coral ecophysiology.

Original languageEnglish (US)
Article number20161003
JournalJournal of the Royal Society Interface
Volume14
Issue number128
DOIs
StatePublished - Mar 1 2017

Fingerprint

Anthozoa
Optical tomography
Optical Coherence Tomography
Skeleton
Tissue
Imaging techniques
Chromatophores
Green Fluorescent Proteins
Pigments
Plasticity
Endoderm
Ectoderm
Visualization
Lighting
Mucus
Infrared radiation
Proteins
Radiation

Keywords

  • Bioimaging
  • Coral tissue
  • Green fluorescent protein
  • Microstructure
  • Optical coherence tomography

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biophysics
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

In vivo imaging of coral tissue and skeleton with optical coherence tomography. / Wangpraseurt, Daniel; Wentzel, Camilla; Jacques, Steven; Wagner, Michael; Kühl, Michael.

In: Journal of the Royal Society Interface, Vol. 14, No. 128, 20161003, 01.03.2017.

Research output: Contribution to journalArticle

Wangpraseurt, Daniel ; Wentzel, Camilla ; Jacques, Steven ; Wagner, Michael ; Kühl, Michael. / In vivo imaging of coral tissue and skeleton with optical coherence tomography. In: Journal of the Royal Society Interface. 2017 ; Vol. 14, No. 128.
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