Abstract
An optical coherence tomography (OCT) system was built to acquire in vivo, both images and vibration measurements of the organ of Corti of the guinea pig. The organ of Corti was viewed through a ~500-μm diameter hole in the bony wall of the scala tympani of the first cochlear turn. In imaging mode, the image was acquired as reflectance R(x,z). In vibration mode, the basilar membrane (BM) or reticular lamina (RL) was selected based on the image. Under software control, the system would move the scanning mirrors to bring the sensing volume of the measurement to the desired tissue location. To address the gain stability problem of the homodyne OCT system, arising from the system moving in and out of the quadrature point and also to resolve the 180 degree ambiguity in the phase measurement using an interferometer, a vibration calibration method is developed by adding a vibrating source to the reference arm to monitor the operating point of the interferometric system. Amplitude gain and phase of various cochlear membranes was measured for different sound pressure level (SPL) varying from 65dB SPL to 93 dB SPL.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6847 |
| DOIs | |
| State | Published - 2008 |
| Event | Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XII - San Jose, CA, United States Duration: Jan 21 2008 → Jan 23 2008 |
Other
| Other | Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XII |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/21/08 → 1/23/08 |
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Keywords
- Cochlea
- Interferometry
- Optical coherence tomography
- Vibration
ASJC Scopus subject areas
- Engineering(all)
Cite this
In vivo imaging and vibration measurement of Guinea pig cochlea. / Choudhury, Niloy; Chen, Fangyi; Zheng, Jiefu; Nuttall, Alfred; Jacques, Steven.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6847 2008. 68471V.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - In vivo imaging and vibration measurement of Guinea pig cochlea
AU - Choudhury, Niloy
AU - Chen, Fangyi
AU - Zheng, Jiefu
AU - Nuttall, Alfred
AU - Jacques, Steven
PY - 2008
Y1 - 2008
N2 - An optical coherence tomography (OCT) system was built to acquire in vivo, both images and vibration measurements of the organ of Corti of the guinea pig. The organ of Corti was viewed through a ~500-μm diameter hole in the bony wall of the scala tympani of the first cochlear turn. In imaging mode, the image was acquired as reflectance R(x,z). In vibration mode, the basilar membrane (BM) or reticular lamina (RL) was selected based on the image. Under software control, the system would move the scanning mirrors to bring the sensing volume of the measurement to the desired tissue location. To address the gain stability problem of the homodyne OCT system, arising from the system moving in and out of the quadrature point and also to resolve the 180 degree ambiguity in the phase measurement using an interferometer, a vibration calibration method is developed by adding a vibrating source to the reference arm to monitor the operating point of the interferometric system. Amplitude gain and phase of various cochlear membranes was measured for different sound pressure level (SPL) varying from 65dB SPL to 93 dB SPL.
AB - An optical coherence tomography (OCT) system was built to acquire in vivo, both images and vibration measurements of the organ of Corti of the guinea pig. The organ of Corti was viewed through a ~500-μm diameter hole in the bony wall of the scala tympani of the first cochlear turn. In imaging mode, the image was acquired as reflectance R(x,z). In vibration mode, the basilar membrane (BM) or reticular lamina (RL) was selected based on the image. Under software control, the system would move the scanning mirrors to bring the sensing volume of the measurement to the desired tissue location. To address the gain stability problem of the homodyne OCT system, arising from the system moving in and out of the quadrature point and also to resolve the 180 degree ambiguity in the phase measurement using an interferometer, a vibration calibration method is developed by adding a vibrating source to the reference arm to monitor the operating point of the interferometric system. Amplitude gain and phase of various cochlear membranes was measured for different sound pressure level (SPL) varying from 65dB SPL to 93 dB SPL.
KW - Cochlea
KW - Interferometry
KW - Optical coherence tomography
KW - Vibration
UR - http://www.scopus.com/inward/record.url?scp=42149183101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149183101&partnerID=8YFLogxK
U2 - 10.1117/12.764663
DO - 10.1117/12.764663
M3 - Conference contribution
SN - 9780819470225
VL - 6847
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -