In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3

A. M. Gronowski, Z. Zhong, Z. Wen, M. J. Thomas, J. E. Darnell, P. Rotwein

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The mechanisms by which GH regulates gene expression to alter growth and metabolism are unknown. We have demonstrated previously that in vivo GH treatment rapidly stimulates the tyrosine phosphorylation of multiple nuclear proteins and have identified the inducible transcription factor Stat1 (formerly Stat91) as one of the major GH-activated nuclear phosphoproteins. We now show that Stat3, a new member of the STAT (signal transducer and activator of transcription) family of transcription factors, is also phosphorylated on tyrosine residues and rapidly appears in the nucleus in response to GH. Activated Stat3 interacts with the naturally occurring c- sis-inducible element of the c-fos gene after GH treatment, as demonstrated by gel mobility shift assay, and is a component of gel-shifted bands A and B when the high affinity sis-inducible element is used as a probe. Our results suggest that multiple STAT proteins may mediate some of the pleiotropic effects of GH on gene expression.

Original languageEnglish (US)
Pages (from-to)171-177
Number of pages7
JournalMolecular Endocrinology
Volume9
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Growth Hormone
Tyrosine
Transcription Factors
Gels
Phosphorylation
STAT Transcription Factors
fos Genes
Gene Expression
Phosphoproteins
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Transducers
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gronowski, A. M., Zhong, Z., Wen, Z., Thomas, M. J., Darnell, J. E., & Rotwein, P. (1995). In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3. Molecular Endocrinology, 9(2), 171-177.

In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3. / Gronowski, A. M.; Zhong, Z.; Wen, Z.; Thomas, M. J.; Darnell, J. E.; Rotwein, P.

In: Molecular Endocrinology, Vol. 9, No. 2, 1995, p. 171-177.

Research output: Contribution to journalArticle

Gronowski, AM, Zhong, Z, Wen, Z, Thomas, MJ, Darnell, JE & Rotwein, P 1995, 'In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3', Molecular Endocrinology, vol. 9, no. 2, pp. 171-177.
Gronowski AM, Zhong Z, Wen Z, Thomas MJ, Darnell JE, Rotwein P. In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3. Molecular Endocrinology. 1995;9(2):171-177.
Gronowski, A. M. ; Zhong, Z. ; Wen, Z. ; Thomas, M. J. ; Darnell, J. E. ; Rotwein, P. / In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3. In: Molecular Endocrinology. 1995 ; Vol. 9, No. 2. pp. 171-177.
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