In vivo ethanol decreases phosphorylated MAPK and p70s6 kinase in the developing rat brain

Ryozo Tsuji, Marina Guizzetti, Lucio G. Costa

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Exposure to ethanol during pregnancy is detrimental to fetal development, and individuals affected by the fetal alcohol syndrome present a number of CN system dysfunctions including microencephaly and mental retardation. Recently, it has been suggested that ethanol-induced inhibition of glial cell proliferation may be relevant in the causation of microencephaly. In this study, we measured the developmental changes of MAPK (ERKI/2) and p70S6 kinase, which are considered to play a prominent role in cell proliferation, and their phosphorylated proteins in rat brain, and examined the effects of in vivo ethanol administration. MAPK and phospho-MAPK increased gradually after birth, and reached adult levels on postnatal day 21. In contrast, levels of both p70S6 kinase and phosphop70S6 kinase decreased after birth. Exposure to ethanol (2-6 g/kg, from postnatal day 4 to 7) had no effects on MAPK or p70S6 kinase levels, but caused a dose-dependent decrease of both phosphoproteins. These results suggest that phosphorylation of MAPK and p70S6 kinase may represent relevant targets for the developmental neurotoxicity of ethanol, and may be involved in microencephaly.

Original languageEnglish (US)
Pages (from-to)1395-1399
Number of pages5
JournalNeuroReport
Volume14
Issue number10
DOIs
StatePublished - Jul 18 2003

Keywords

  • Developmental neurotoxicity
  • Ethanol
  • Fetal alcohol syndrome
  • MAPK
  • Microencephaly
  • p70S6 kinase

ASJC Scopus subject areas

  • Neuroscience(all)

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