In vivo effect of calcitriol on calcium transport and calcium binding proteins in the spontaneously hypertensive rat

Chantal M. Roullet, Jean-Baptiste Roullet, Anne Sophie Martin, David A. McCarron

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The abnormal intestinal Ca2+ transport reported in spontaneously hypertensive rats (SHR) has been attributed to decreased responsiveness to calcitriol. We reexamined this hypothesis by studying the calcitriol regulation of SHR duodenal calbindin-D9K and calmodulin and the relation of calcitriol to Ca2+ uptake by isolated enterocytes. SHR and normotensive Wistar-Kyoto (WKY) rats were injected with either 50 ng/d calcitriol (vit-D) or vehicle alone (control) for 3 days. Decreased calbindin-D9K (P2+ flux (P2+ flux, which increased in vit-D animals, remained lower in SHR for plasma calcitriol levels similar to those in WKY rats. Immunoreactive calmodulin was similar in both strains whether assayed in total cell or brush border membranes. In contrast, when measured by ligand blotting (45Ca), calmodulin was lower in SHR than in WKY rats (P2+ binding capacity in the hypertensive strain. Calcitriol had no effect on calmodulin in either strain. In conclusion, Ca2+ binding protein regulation by calcitriol is normal in the SHR, and decreased hormone responsiveness cannot account for the defective duodenal calcium transport of this experimental model of hypertension.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalHypertension
Volume24
Issue number2
StatePublished - Aug 1994

Fingerprint

Calcium-Binding Proteins
Calcitriol
Inbred SHR Rats
Calcium
Calmodulin
Inbred WKY Rats
S100 Calcium Binding Protein G
Enterocytes
Microvilli
Carrier Proteins
Theoretical Models
Hormones
Ligands
Hypertension
Membranes

Keywords

  • calcitriol
  • calcium binding proteins
  • calmodulin
  • rats, inbred SHR

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Roullet, C. M., Roullet, J-B., Martin, A. S., & McCarron, D. A. (1994). In vivo effect of calcitriol on calcium transport and calcium binding proteins in the spontaneously hypertensive rat. Hypertension, 24(2), 176-182.

In vivo effect of calcitriol on calcium transport and calcium binding proteins in the spontaneously hypertensive rat. / Roullet, Chantal M.; Roullet, Jean-Baptiste; Martin, Anne Sophie; McCarron, David A.

In: Hypertension, Vol. 24, No. 2, 08.1994, p. 176-182.

Research output: Contribution to journalArticle

Roullet, CM, Roullet, J-B, Martin, AS & McCarron, DA 1994, 'In vivo effect of calcitriol on calcium transport and calcium binding proteins in the spontaneously hypertensive rat', Hypertension, vol. 24, no. 2, pp. 176-182.
Roullet, Chantal M. ; Roullet, Jean-Baptiste ; Martin, Anne Sophie ; McCarron, David A. / In vivo effect of calcitriol on calcium transport and calcium binding proteins in the spontaneously hypertensive rat. In: Hypertension. 1994 ; Vol. 24, No. 2. pp. 176-182.
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