TY - JOUR
T1 - In vivo characterization of the proteasome regulator PA28
AU - Ahn, Kwangseog
AU - Erlander, Mark
AU - Leturcq, Didier
AU - Peterson, Per A.
AU - Früh, Klaus
AU - Yang, Young
PY - 1996
Y1 - 1996
N2 - A proteasome regulator, termed PA28, has been shown to modulate peptidase activities of the proteasomes in vitro. Two different but homologous PA28 molecules, designated as PA28α and PA28β, have been cloned. Both a and β polypeptides of PA28 are found in PA28 complexes isolated from cells, indicating that both are constituents of functional PA28 complexes. Using antisera specific to pA28α, PA28β, and epitope-tagged PA28 molecules, we show that expression of PA28α and PA28β is coordinately induced by various cytokines in different cell lines and that PA28 subunits and proteasomes have almost identical half-lives. In addition, we show that PA28 complexes are associated with 20 S but not 26 S proteasomes in vivo. Moreover, we demonstrate that PA28 complex is a heterohexamer composed of both α and β subunits with a stoichiometry of α3β3 in an alternating order.
AB - A proteasome regulator, termed PA28, has been shown to modulate peptidase activities of the proteasomes in vitro. Two different but homologous PA28 molecules, designated as PA28α and PA28β, have been cloned. Both a and β polypeptides of PA28 are found in PA28 complexes isolated from cells, indicating that both are constituents of functional PA28 complexes. Using antisera specific to pA28α, PA28β, and epitope-tagged PA28 molecules, we show that expression of PA28α and PA28β is coordinately induced by various cytokines in different cell lines and that PA28 subunits and proteasomes have almost identical half-lives. In addition, we show that PA28 complexes are associated with 20 S but not 26 S proteasomes in vivo. Moreover, we demonstrate that PA28 complex is a heterohexamer composed of both α and β subunits with a stoichiometry of α3β3 in an alternating order.
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U2 - 10.1074/jbc.271.30.18237
DO - 10.1074/jbc.271.30.18237
M3 - Article
C2 - 8663520
AN - SCOPUS:0030014843
SN - 0021-9258
VL - 271
SP - 18237
EP - 18242
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 30
ER -