In vivo assessment of basal and drug‐induced dopamine release in cortical and subcortical regions of the anesthetized primate

Bita Moghaddam, Craig W. Berridge, Patricia S. Goldman‐Rakic, Benjamin S. Bunney, Robert H. Roth

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

There is an acute interest in studying the functional characteristics of dopamine systems in the cortex of primates. In particular, the prefrontal cortical dopamine projections have received a great deal of attention. This system is essential for proper functioning of the prefrontal cortex, and dysfunction within the system may be involved in some psychiatric and neurological illnesses. In vivo assessments of cortical dopamine in the primate have been scarce. This has been due, in part, to technical difficulties associated with these studies and with quantifying the relatively low levels of dopamine found in cortical regions. In the present study, intracerebral microdialysis was utilized to assess the extracellular concentration of dopamine in cortical and subcortical areas of the pentobarbital‐anesthetized rhesus monkey. Basal extracellular dopamine levels were consistently detected in the medial prefrontal cortex, premotor cortex, and caudate‐putamen. The basal extracellular concentration of dopamine in the dorsolateral prefrontal cortex was reliably detected in 1 of 4 animals. Intravenous administration of amphetamine (1 mg/kg) enhanced extracellular dopamine levels in the caudate‐putamen area by more than 20‐fold. In cortical areas, amphetamine's effect was less profound: An increase of 400–500 percent over basal extracellular dopamine levels was observed in each region. These studies demonstrate the feasibility of microdialysis for detecting extracellular fluxes of dopamine in the cortex of nonhuman primates. They further provide direct evidence that the dopamine released within the prefrontal cortex and the premotor cortex of nonhuman primates responds to pharmacological manipulation. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalSynapse
Volume13
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Primates
Dopamine
Prefrontal Cortex
Microdialysis
Motor Cortex
Feasibility Studies
Amphetamine
Macaca mulatta
Intravenous Administration
Psychiatry
Pharmacology

Keywords

  • Amphetamine
  • Caudate‐putamen
  • Dopamine
  • Microdialysis
  • Prefrontal cortex
  • Premotor cortex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

In vivo assessment of basal and drug‐induced dopamine release in cortical and subcortical regions of the anesthetized primate. / Moghaddam, Bita; Berridge, Craig W.; Goldman‐Rakic, Patricia S.; Bunney, Benjamin S.; Roth, Robert H.

In: Synapse, Vol. 13, No. 3, 1993, p. 215-222.

Research output: Contribution to journalArticle

Moghaddam, Bita ; Berridge, Craig W. ; Goldman‐Rakic, Patricia S. ; Bunney, Benjamin S. ; Roth, Robert H. / In vivo assessment of basal and drug‐induced dopamine release in cortical and subcortical regions of the anesthetized primate. In: Synapse. 1993 ; Vol. 13, No. 3. pp. 215-222.
@article{47bac8a1f0a348f191e7a5021b8fb832,
title = "In vivo assessment of basal and drug‐induced dopamine release in cortical and subcortical regions of the anesthetized primate",
abstract = "There is an acute interest in studying the functional characteristics of dopamine systems in the cortex of primates. In particular, the prefrontal cortical dopamine projections have received a great deal of attention. This system is essential for proper functioning of the prefrontal cortex, and dysfunction within the system may be involved in some psychiatric and neurological illnesses. In vivo assessments of cortical dopamine in the primate have been scarce. This has been due, in part, to technical difficulties associated with these studies and with quantifying the relatively low levels of dopamine found in cortical regions. In the present study, intracerebral microdialysis was utilized to assess the extracellular concentration of dopamine in cortical and subcortical areas of the pentobarbital‐anesthetized rhesus monkey. Basal extracellular dopamine levels were consistently detected in the medial prefrontal cortex, premotor cortex, and caudate‐putamen. The basal extracellular concentration of dopamine in the dorsolateral prefrontal cortex was reliably detected in 1 of 4 animals. Intravenous administration of amphetamine (1 mg/kg) enhanced extracellular dopamine levels in the caudate‐putamen area by more than 20‐fold. In cortical areas, amphetamine's effect was less profound: An increase of 400–500 percent over basal extracellular dopamine levels was observed in each region. These studies demonstrate the feasibility of microdialysis for detecting extracellular fluxes of dopamine in the cortex of nonhuman primates. They further provide direct evidence that the dopamine released within the prefrontal cortex and the premotor cortex of nonhuman primates responds to pharmacological manipulation. {\circledC} 1993 Wiley‐Liss, Inc.",
keywords = "Amphetamine, Caudate‐putamen, Dopamine, Microdialysis, Prefrontal cortex, Premotor cortex",
author = "Bita Moghaddam and Berridge, {Craig W.} and Goldman‐Rakic, {Patricia S.} and Bunney, {Benjamin S.} and Roth, {Robert H.}",
year = "1993",
doi = "10.1002/syn.890130304",
language = "English (US)",
volume = "13",
pages = "215--222",
journal = "Synapse",
issn = "0887-4476",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - In vivo assessment of basal and drug‐induced dopamine release in cortical and subcortical regions of the anesthetized primate

AU - Moghaddam, Bita

AU - Berridge, Craig W.

AU - Goldman‐Rakic, Patricia S.

AU - Bunney, Benjamin S.

AU - Roth, Robert H.

PY - 1993

Y1 - 1993

N2 - There is an acute interest in studying the functional characteristics of dopamine systems in the cortex of primates. In particular, the prefrontal cortical dopamine projections have received a great deal of attention. This system is essential for proper functioning of the prefrontal cortex, and dysfunction within the system may be involved in some psychiatric and neurological illnesses. In vivo assessments of cortical dopamine in the primate have been scarce. This has been due, in part, to technical difficulties associated with these studies and with quantifying the relatively low levels of dopamine found in cortical regions. In the present study, intracerebral microdialysis was utilized to assess the extracellular concentration of dopamine in cortical and subcortical areas of the pentobarbital‐anesthetized rhesus monkey. Basal extracellular dopamine levels were consistently detected in the medial prefrontal cortex, premotor cortex, and caudate‐putamen. The basal extracellular concentration of dopamine in the dorsolateral prefrontal cortex was reliably detected in 1 of 4 animals. Intravenous administration of amphetamine (1 mg/kg) enhanced extracellular dopamine levels in the caudate‐putamen area by more than 20‐fold. In cortical areas, amphetamine's effect was less profound: An increase of 400–500 percent over basal extracellular dopamine levels was observed in each region. These studies demonstrate the feasibility of microdialysis for detecting extracellular fluxes of dopamine in the cortex of nonhuman primates. They further provide direct evidence that the dopamine released within the prefrontal cortex and the premotor cortex of nonhuman primates responds to pharmacological manipulation. © 1993 Wiley‐Liss, Inc.

AB - There is an acute interest in studying the functional characteristics of dopamine systems in the cortex of primates. In particular, the prefrontal cortical dopamine projections have received a great deal of attention. This system is essential for proper functioning of the prefrontal cortex, and dysfunction within the system may be involved in some psychiatric and neurological illnesses. In vivo assessments of cortical dopamine in the primate have been scarce. This has been due, in part, to technical difficulties associated with these studies and with quantifying the relatively low levels of dopamine found in cortical regions. In the present study, intracerebral microdialysis was utilized to assess the extracellular concentration of dopamine in cortical and subcortical areas of the pentobarbital‐anesthetized rhesus monkey. Basal extracellular dopamine levels were consistently detected in the medial prefrontal cortex, premotor cortex, and caudate‐putamen. The basal extracellular concentration of dopamine in the dorsolateral prefrontal cortex was reliably detected in 1 of 4 animals. Intravenous administration of amphetamine (1 mg/kg) enhanced extracellular dopamine levels in the caudate‐putamen area by more than 20‐fold. In cortical areas, amphetamine's effect was less profound: An increase of 400–500 percent over basal extracellular dopamine levels was observed in each region. These studies demonstrate the feasibility of microdialysis for detecting extracellular fluxes of dopamine in the cortex of nonhuman primates. They further provide direct evidence that the dopamine released within the prefrontal cortex and the premotor cortex of nonhuman primates responds to pharmacological manipulation. © 1993 Wiley‐Liss, Inc.

KW - Amphetamine

KW - Caudate‐putamen

KW - Dopamine

KW - Microdialysis

KW - Prefrontal cortex

KW - Premotor cortex

UR - http://www.scopus.com/inward/record.url?scp=0027407724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027407724&partnerID=8YFLogxK

U2 - 10.1002/syn.890130304

DO - 10.1002/syn.890130304

M3 - Article

C2 - 8497807

AN - SCOPUS:0027407724

VL - 13

SP - 215

EP - 222

JO - Synapse

JF - Synapse

SN - 0887-4476

IS - 3

ER -