In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster

Research output: Contribution to journalArticle

Abstract

Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

Original languageEnglish (US)
Pages (from-to)321-338
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume2143
DOIs
StatePublished - Jan 1 2020

Keywords

  • Axotomy
  • Drosophila
  • Glia
  • Glial immunity
  • Neurodegeneration
  • Phagocytosis
  • Wallerian degeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this