In vivo analysis of glial immune responses to axon degeneration in Drosophila melanogaster

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages321-338
Number of pages18
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2143
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Axotomy
  • Drosophila
  • Glia
  • Glial immunity
  • Neurodegeneration
  • Phagocytosis
  • Wallerian degeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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