In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem

Craig R. Smith, Andrés Jaramillo, Nancy J. Poindexter, Nancy S. Steward, Kim Lu, Daniel C. Brennan, Gary G. Singer, Brent W. Miller, Martin D. Jendrisak, Surendra Shenoy, Jeffrey A. Lowell, Todd K. Howard, T. Mohanakumar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Acute rejection of renal allografts is mediated by infiltrating alloreactive T cells. The goals of this study were to correlate T cell proliferation with rejection and to determine whether T cell proliferation in the absence of rejection would predict future rejection episodes. Toward this, kidney biopsies (n=100) were cultured in the presence of interleukin-2. Cultures were examined at 4, 24, and 48 hr for T cell proliferation. A strong correlation was observed between T cell proliferation at any time point and rejection. There was not a significant correlation between T cell proliferation in biopsies with no rejection and the occurrence of a rejection episode within 2 months. However, T cell proliferation after 4 hr was a better predictor of the occurrence of rejection within 2 months compared with observations after 24 and 48 hr. Therefore, a subgroup of patients with unremarkable biopsies but T cell proliferation may be at risk for rejection and warrant closer observation and possible tailoring of immunosuppression.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalTransplantation
Volume73
Issue number1
StatePublished - Jan 15 2002
Externally publishedYes

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Allografts
Cell Proliferation
Pathology
T-Lymphocytes
Kidney
Biopsy
In Vitro Techniques
Immunosuppression
Interleukin-2
Observation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Smith, C. R., Jaramillo, A., Poindexter, N. J., Steward, N. S., Lu, K., Brennan, D. C., ... Mohanakumar, T. (2002). In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem. Transplantation, 73(1), 142-145.

In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology : New strategies for an old problem. / Smith, Craig R.; Jaramillo, Andrés; Poindexter, Nancy J.; Steward, Nancy S.; Lu, Kim; Brennan, Daniel C.; Singer, Gary G.; Miller, Brent W.; Jendrisak, Martin D.; Shenoy, Surendra; Lowell, Jeffrey A.; Howard, Todd K.; Mohanakumar, T.

In: Transplantation, Vol. 73, No. 1, 15.01.2002, p. 142-145.

Research output: Contribution to journalArticle

Smith, CR, Jaramillo, A, Poindexter, NJ, Steward, NS, Lu, K, Brennan, DC, Singer, GG, Miller, BW, Jendrisak, MD, Shenoy, S, Lowell, JA, Howard, TK & Mohanakumar, T 2002, 'In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem', Transplantation, vol. 73, no. 1, pp. 142-145.
Smith CR, Jaramillo A, Poindexter NJ, Steward NS, Lu K, Brennan DC et al. In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem. Transplantation. 2002 Jan 15;73(1):142-145.
Smith, Craig R. ; Jaramillo, Andrés ; Poindexter, Nancy J. ; Steward, Nancy S. ; Lu, Kim ; Brennan, Daniel C. ; Singer, Gary G. ; Miller, Brent W. ; Jendrisak, Martin D. ; Shenoy, Surendra ; Lowell, Jeffrey A. ; Howard, Todd K. ; Mohanakumar, T. / In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology : New strategies for an old problem. In: Transplantation. 2002 ; Vol. 73, No. 1. pp. 142-145.
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