In vitro replicative arrest in dnaAts mutants

Musetta A. Hanson, Robb E. Moses

Research output: Contribution to journalArticle

Abstract

Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaAts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume190
Issue number2
DOIs
StatePublished - Oct 1978

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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