Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaAts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.
ASJC Scopus subject areas
- Molecular Biology