In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid

Vincent M. Rossi, Bradley M. White, Mariko J. Newton, Steven Jacques, Paige J. Baugher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This is an in vitro study of photodynamic therapy (PDT) in the MG-63 line of human osteosarcoma cells, as mediated by aminolevulinic acid (ALA). The primary goal of this work is to determine the feasibility and effectiveness of treating osteosarcoma through PDT. In addition, this work is aimed at determining whether the resulting cell death occurs through apoptosis or cellular necrosis. The MG-63 cells are treated with increasing concentrations of ALA from 0.1-10 mM ALA, leading to the accumulation of the photosensitizer protoporphyrin IX (PpIX) within the cells. After incubation periods of 4 and 24 hours in ALA, the cells are illuminated by 0-10 J/cm2 of 636 nm light in order to activate the PpIX and induce oxidative damage to the cells. Light is administered by an 8x12 array of LED's, which are controlled by an Arduino Duemilanove microcontroller board in order to assure ease of use along with accurate levels of exposure. Controls for this experiment include 0 J/cm 2 of light exposure for all experimental concentrations of ALA, as well as illuminating cells that have not been incubated in ALA at all experimental levels of illumination. MG-63 cells are analyzed through fluorimetry and MTT assays in order to determine the effectiveness of ALA mediated PDT of osteosarcoma.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7886
DOIs
StatePublished - 2011
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 23 2011

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/23/11

Fingerprint

Aminolevulinic Acid
Photodynamic therapy
Photochemotherapy
Osteosarcoma
therapy
acids
Acids
cells
Photosensitizing Agents
Photosensitizers
Cell death
Light
Fluorometry
necrosis
apoptosis
Microcontrollers
In Vitro Techniques
Lighting
death
illuminating

Keywords

  • ALA
  • LED array
  • MG-63 cells
  • Monte Carlo
  • PDT
  • PpIX. osteosarcoma

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Rossi, V. M., White, B. M., Newton, M. J., Jacques, S., & Baugher, P. J. (2011). In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7886). [78860T] https://doi.org/10.1117/12.876337

In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. / Rossi, Vincent M.; White, Bradley M.; Newton, Mariko J.; Jacques, Steven; Baugher, Paige J.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7886 2011. 78860T.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rossi, VM, White, BM, Newton, MJ, Jacques, S & Baugher, PJ 2011, In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7886, 78860T, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.876337
Rossi VM, White BM, Newton MJ, Jacques S, Baugher PJ. In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7886. 2011. 78860T https://doi.org/10.1117/12.876337
Rossi, Vincent M. ; White, Bradley M. ; Newton, Mariko J. ; Jacques, Steven ; Baugher, Paige J. / In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7886 2011.
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