In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid

Vincent M. Rossi, Bradley M. White, Mariko J. Newton, Steven L. Jacques, Paige J. Baugher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

This is an in vitro study of photodynamic therapy (PDT) in the MG-63 line of human osteosarcoma cells, as mediated by aminolevulinic acid (ALA). The primary goal of this work is to determine the feasibility and effectiveness of treating osteosarcoma through PDT. In addition, this work is aimed at determining whether the resulting cell death occurs through apoptosis or cellular necrosis. The MG-63 cells are treated with increasing concentrations of ALA from 0.1-10 mM ALA, leading to the accumulation of the photosensitizer protoporphyrin IX (PpIX) within the cells. After incubation periods of 4 and 24 hours in ALA, the cells are illuminated by 0-10 J/cm2 of 636 nm light in order to activate the PpIX and induce oxidative damage to the cells. Light is administered by an 8x12 array of LED's, which are controlled by an Arduino Duemilanove microcontroller board in order to assure ease of use along with accurate levels of exposure. Controls for this experiment include 0 J/cm 2 of light exposure for all experimental concentrations of ALA, as well as illuminating cells that have not been incubated in ALA at all experimental levels of illumination. MG-63 cells are analyzed through fluorimetry and MTT assays in order to determine the effectiveness of ALA mediated PDT of osteosarcoma.

Original languageEnglish (US)
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XX
DOIs
StatePublished - Apr 20 2011
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 23 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7886
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/23/11

Keywords

  • ALA
  • LED array
  • MG-63 cells
  • Monte Carlo
  • PDT
  • PpIX. osteosarcoma

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Rossi, V. M., White, B. M., Newton, M. J., Jacques, S. L., & Baugher, P. J. (2011). In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX [78860T] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7886). https://doi.org/10.1117/12.876337