In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid

Vincent M. Rossi; Bradley M. White; Mariko J. Newton; Steven L. Jacques; Paige J. Baugher

doi:10.1117/12.876337

In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid

Vincent M. Rossi, Bradley M. White, Mariko J. Newton, Steven L. Jacques, Paige J. Baugher

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Scopus citations

Abstract

This is an in vitro study of photodynamic therapy (PDT) in the MG-63 line of human osteosarcoma cells, as mediated by aminolevulinic acid (ALA). The primary goal of this work is to determine the feasibility and effectiveness of treating osteosarcoma through PDT. In addition, this work is aimed at determining whether the resulting cell death occurs through apoptosis or cellular necrosis. The MG-63 cells are treated with increasing concentrations of ALA from 0.1-10 mM ALA, leading to the accumulation of the photosensitizer protoporphyrin IX (PpIX) within the cells. After incubation periods of 4 and 24 hours in ALA, the cells are illuminated by 0-10 J/cm² of 636 nm light in order to activate the PpIX and induce oxidative damage to the cells. Light is administered by an 8x12 array of LED's, which are controlled by an Arduino Duemilanove microcontroller board in order to assure ease of use along with accurate levels of exposure. Controls for this experiment include 0 J/cm ² of light exposure for all experimental concentrations of ALA, as well as illuminating cells that have not been incubated in ALA at all experimental levels of illumination. MG-63 cells are analyzed through fluorimetry and MTT assays in order to determine the effectiveness of ALA mediated PDT of osteosarcoma.

Original language	English (US)
Title of host publication	Optical Methods for Tumor Treatment and Detection
Subtitle of host publication	Mechanisms and Techniques in Photodynamic Therapy XX
DOIs	https://doi.org/10.1117/12.876337
State	Published - 2011
Externally published	Yes
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX - San Francisco, CA, United States Duration: Jan 22 2011 → Jan 23 2011

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7886
ISSN (Print)	1605-7422

Other

Other	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX
Country/Territory	United States
City	San Francisco, CA
Period	1/22/11 → 1/23/11

Keywords

ALA
LED array
MG-63 cells
Monte Carlo
PDT
PpIX. osteosarcoma

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging
Biomaterials

Access to Document

10.1117/12.876337

Cite this

Rossi, V. M., White, B. M., Newton, M. J., Jacques, S. L., & Baugher, P. J. (2011). In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX Article 78860T (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7886). https://doi.org/10.1117/12.876337

In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. / Rossi, Vincent M.; White, Bradley M.; Newton, Mariko J. et al.
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX. 2011. 78860T (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7886).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Rossi, VM, White, BM, Newton, MJ, Jacques, SL & Baugher, PJ 2011, In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid. in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX., 78860T, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.876337

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abstract = "This is an in vitro study of photodynamic therapy (PDT) in the MG-63 line of human osteosarcoma cells, as mediated by aminolevulinic acid (ALA). The primary goal of this work is to determine the feasibility and effectiveness of treating osteosarcoma through PDT. In addition, this work is aimed at determining whether the resulting cell death occurs through apoptosis or cellular necrosis. The MG-63 cells are treated with increasing concentrations of ALA from 0.1-10 mM ALA, leading to the accumulation of the photosensitizer protoporphyrin IX (PpIX) within the cells. After incubation periods of 4 and 24 hours in ALA, the cells are illuminated by 0-10 J/cm2 of 636 nm light in order to activate the PpIX and induce oxidative damage to the cells. Light is administered by an 8x12 array of LED's, which are controlled by an Arduino Duemilanove microcontroller board in order to assure ease of use along with accurate levels of exposure. Controls for this experiment include 0 J/cm 2 of light exposure for all experimental concentrations of ALA, as well as illuminating cells that have not been incubated in ALA at all experimental levels of illumination. MG-63 cells are analyzed through fluorimetry and MTT assays in order to determine the effectiveness of ALA mediated PDT of osteosarcoma.",

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AB - This is an in vitro study of photodynamic therapy (PDT) in the MG-63 line of human osteosarcoma cells, as mediated by aminolevulinic acid (ALA). The primary goal of this work is to determine the feasibility and effectiveness of treating osteosarcoma through PDT. In addition, this work is aimed at determining whether the resulting cell death occurs through apoptosis or cellular necrosis. The MG-63 cells are treated with increasing concentrations of ALA from 0.1-10 mM ALA, leading to the accumulation of the photosensitizer protoporphyrin IX (PpIX) within the cells. After incubation periods of 4 and 24 hours in ALA, the cells are illuminated by 0-10 J/cm2 of 636 nm light in order to activate the PpIX and induce oxidative damage to the cells. Light is administered by an 8x12 array of LED's, which are controlled by an Arduino Duemilanove microcontroller board in order to assure ease of use along with accurate levels of exposure. Controls for this experiment include 0 J/cm 2 of light exposure for all experimental concentrations of ALA, as well as illuminating cells that have not been incubated in ALA at all experimental levels of illumination. MG-63 cells are analyzed through fluorimetry and MTT assays in order to determine the effectiveness of ALA mediated PDT of osteosarcoma.

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In vitro photodynamic therapy of MG-63 osteosarcoma cells mediated by aminolevulinic acid

Abstract

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