In vitro method for measuring synthesis rates in the intervertebral disc

M. T. Bayliss, J. P G Urban, Brian Johnstone, S. Holm

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

A method for culturing intervertebral disc tissue in vitro under conditions that control changes in hydration and minimize loss of proteoglycans has been developed. Disc slices were enclosed in small-pore dialysis tubing and incubated in medium containing polyethylene glycol (PEG). By varying the PEG concentration, different external swelling pressures could be applied to the tissue. The rate of glycosaminoglycan synthesis in rabbit and dog disc was then measured using left bracket **3**5S right bracket sulfate as a precursor. Synthesis rates varied with final hydration of the tissue, and the highest rates were found at hydrations close to those found in vivo. under conditions of controlled hydration, rabbit nucleus has a higher rate of glycosaminoglycan synthesis than annulus. However, the rates measured in sagittal slices of dog disc decreased from the outer to the inner regions of the anterior annulus, reaching a minimum value in the nucleus and then increasing again in the posterior part of the annulus. The results show that in the intervertebral disc, measured rates depend on culture conditions.

Original languageEnglish (US)
Pages (from-to)10-17
Number of pages8
JournalJournal of Orthopaedic Research
Volume4
Issue number1
StatePublished - 1986
Externally publishedYes

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Intervertebral Disc
Glycosaminoglycans
Dogs
Rabbits
Proteoglycans
Sulfates
Dialysis
Pressure
In Vitro Techniques

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

In vitro method for measuring synthesis rates in the intervertebral disc. / Bayliss, M. T.; Urban, J. P G; Johnstone, Brian; Holm, S.

In: Journal of Orthopaedic Research, Vol. 4, No. 1, 1986, p. 10-17.

Research output: Contribution to journalArticle

Bayliss, M. T. ; Urban, J. P G ; Johnstone, Brian ; Holm, S. / In vitro method for measuring synthesis rates in the intervertebral disc. In: Journal of Orthopaedic Research. 1986 ; Vol. 4, No. 1. pp. 10-17.
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